ArticleDevelopmental biology2009
The Galpha4 G protein subunit interacts with the MAP kinase ERK2 using a D-motif that regulates developmental morphogenesis in Dictyostelium.
Article in Developmental biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- An integrated, cross-regulation pathway model involving activating/adaptive and feed-forward/feed-back loops for directed oscillatory cAMP signal-relay/response during the development ofFrontiers in cell and developmental biology · 2023Article
- Host-Induced Gene Silencing of a G Protein α Subunit GeneJournal of fungi (Basel, Switzerland) · 2021Article
- MAPK docking motif in the Dictyostelium Gα2 subunit is required for aggregation and transcription factor translocation.Cellular signalling · 2021Article
- Dictyostelium Erk2 is an atypical MAPK required for chemotaxis.Cellular signalling · 2018Article
- Article
- A GPCR Handles Bacterial Sensing in Chemotaxis and Phagocytosis.Developmental cell · 2016Article
- Pheromone-induced morphogenesis and gradient tracking are dependent on the MAPK Fus3 binding to Gα.Molecular biology of the cell · 2015Article
- The Dictyostelium MAPK ERK1 is phosphorylated in a secondary response to early developmental signaling.Cellular signalling · 2015Article
- Mef2A, a homologue of animal Mef2 transcription factors, regulates cell differentiation in Dictyostelium discoideum.BMC developmental biology · 2013Article
- MAPKs in development: insights from Dictyostelium signaling pathways.Biomolecular concepts · 2011Article
- MAP kinases have different functions in Dictyostelium G protein-mediated signaling.Cellular signalling · 2010Article
- G{alpha}5 subunit-mediated signalling requires a D-motif and the MAPK ERK1 in Dictyostelium.Microbiology (Reading, England) · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
G protein Galpha subunits contribute to the specificity of different signal transduction pathways in Dictyostelium discoideum but Galpha subunit-effector interactions have not been previously identified. The requirement of the Dictyostelium Galpha4 subunit for MAP kinase (MAPK) activation and the identification of a putative MAPK docking site (D-motif) in this subunit suggested a possible interaction between the Galpha4 subunit and MAPKs. In vivo association of the Galpha4 subunit and ERK2 was demonstrated by pull-down and co-immunoprecipitation assays. Alteration of the D-motif reduced Galpha4 subunit-ERK2 interactions but only slightly altered MAPK activation in response to folate. Expression of the Galpha4 subunit with the altered D-motif in galpha4(-)cells allowed for slug formation but not the morphogenesis associated with culmination. Expression of this mutant Galpha4 subunit was sufficient to rescue chemotactic movement to folate. Alteration of the D-motif also reduced the aggregation defect associated with constitutively active Galpha4 subunits. These results suggest Galpha4 subunit-MAPK interactions are necessary for developmental morphogenesis but not for chemotaxis to folate.
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