ReviewParasitology2010
Culture for genetic manipulation of developmental stages of Schistosoma mansoni.
Review in Parasitology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 115 citations in OpenAlex.
- Article
- Viruses of parasites: A roadmap toward diagnostic and therapeutic development.PLoS neglected tropical diseases · 2025Review
- A single-cell atlas of the miracidium larva ofeLife · 2024Article
- Single-cell transcriptomics of the human parasite Schistosoma mansoni first intra-molluscan stage reveals tentative tegumental and stem-cell regulators.Scientific reports · 2024Article
- An improved medium for in vitro studies of female reproduction and oviposition in Schistosoma japonicum.Parasites & vectors · 2024Article
- The stage- and sex-specific transcriptome of the human parasite Schistosoma mansoni.Scientific data · 2023Article
- The Human Blood Fluke, Schistosoma mansoni, Harbors Bacteria Throughout the Parasite's Life Cycle.The Journal of infectious diseases · 2023Article
- Oncolytic Myxoma virus infects and damages the tegument of the human parasitic flatworm Schistosoma mansoni.Experimental parasitology · 2022Article
- Excretion patterns ofParasitology · 2022Article
- RNA-GuidedInternational journal of molecular sciences · 2022Article
- Common aquatic pollutants modify hemocyte immune responses inFrontiers in immunology · 2022Article
- The Transcriptome ofFrontiers in tropical diseases · 2021Article
- Schistosoma mansoni Eggs Modulate the Timing of Granuloma Formation to Promote Transmission.Cell host & microbe · 2021Article
- Urogenital Schistosomiasis-History, Pathogenesis, and Bladder Cancer.Journal of clinical medicine · 2021Review
- Article
- Helminth Microbiota Profiling Using Bacterial 16S rRNA Gene Amplicon Sequencing: From Sampling to Sequence Data Mining.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Single-cell atlas of the first intra-mammalian developmental stage of the human parasite Schistosoma mansoni.Nature communications · 2020Article
- A bug's life: Delving into the challenges of helminth microbiome studies.PLoS neglected tropical diseases · 2020Review
- Large CRISPR-Cas-induced deletions in the oxamniquine resistance locus of the human parasiteWellcome open research · 2020Article
- Myricetin Possesses Anthelmintic Activity and Attenuates Hepatic FibrosisFrontiers in immunology · 2020Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Genomes of the major human helminth parasites, and indeed many others of agricultural significance, are now the research focus of intensive genome sequencing and annotation. A draft genome sequence of the filarial parasite Brugia malayi was reported in 2007 and draft genomes of two of the human schistosomes, Schistosoma japonicum and S. mansoni reported in 2009. These genome data provide the basis for a comprehensive understanding of the molecular mechanisms involved in schistosome nutrition and metabolism, host-dependent development and maturation, immune evasion and invertebrate evolution. In addition, new potential vaccine candidates and drug targets will likely be predicted. However, testing these predictions is often not straightforward with schistosomes because of the difficulty and expense in maintenance of the developmental cycle. To facilitate this goal, several developmental stages can be maintained in vitro for shorter or longer intervals of time, and these are amenable to manipulation. Our research interests focus on experimental studies of schistosome gene functions, and more recently have focused on development of transgenesis and RNA interference with the longer term aim of heritable gene manipulation. Here we review methods to isolate and culture developmental stages of Schistosoma mansoni, including eggs, sporocysts, schistosomules and adults, in particular as these procedures relate to approaches for gene manipulation. We also discuss recent advances in genetic manipulation of schistosomes including the deployment of square wave electroporation to introduce reporter genes into cultured schistosomes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.