ArticleBMC cancer2009
Proliferative and anti-proliferative effects of dietary levels of phytoestrogens in rat pituitary GH3/B6/F10 cells - the involvement of rapidly activated kinases and caspases.
Article in BMC cancer, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Bisphenol A induces human uterine leiomyoma cell proliferation through membrane-associated ERα36 via nongenomic signaling pathways.Molecular and cellular endocrinology · 2019Article
- Xenoestrogen interference with nongenomic signaling actions of physiological estrogens in endocrine cancer cells.Steroids · 2019Article
- Effect of Genistein and 17-β Estradiol on the Viability and Apoptosis of Human Hepatocellular Carcinoma HepG2 cell line.Advanced biomedical research · 2017Article
- Coumestrol inhibits autoantibody production through modulating Th1 response in experimental autoimmune thyroiditis.Oncotarget · 2016Article
- Direct estradiol and diethylstilbestrol actions on early- versus late-stage prostate cancer cells.The Prostate · 2014Article
- Article
- Multi-well plate immunoassays for measuring signaling protein activations/deactivations and membrane vs. intracellular receptor levels.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Mixtures of xenoestrogens disrupt estradiol-induced non-genomic signaling and downstream functions in pituitary cells.Environmental health : a global access science source · 2013Article
- Bisphenol S disrupts estradiol-induced nongenomic signaling in a rat pituitary cell line: effects on cell functions.Environmental health perspectives · 2013Article
- Non-genomic effects of xenoestrogen mixtures.International journal of environmental research and public health · 2012Review
- Article
- Quantitative changes in intracellular calcium and extracellular-regulated kinase activation measured in parallel in CHO cells stably expressing serotonin (5-HT) 5-HT2A or 5-HT2C receptors.BMC neuroscience · 2012Article
- Endocrine disruption via estrogen receptors that participate in nongenomic signaling pathways.The Journal of steroid biochemistry and molecular biology · 2011Review
- Combinations of physiologic estrogens with xenoestrogens alter ERK phosphorylation profiles in rat pituitary cells.Environmental health perspectives · 2011Article
- Combinations of physiologic estrogens with xenoestrogens alter calcium and kinase responses, prolactin release, and membrane estrogen receptor trafficking in rat pituitary cells.Environmental health : a global access science source · 2010Article
- Individual and combined soy isoflavones exert differential effects on metastatic cancer progression.Clinical & experimental metastasis · 2010Article
- Estrogens of multiple classes and their role in mental health disease mechanisms.International journal of women's health · 2010Article
- Subchronic exposure to phytoestrogens alone and in combination with diethylstilbestrol - pituitary tumor induction in Fischer 344 rats.Nutrition & metabolism · 2010Article
- Nongenomic signaling pathways of estrogen toxicity.Toxicological sciences : an official journal of the Society of Toxicology · 2010Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhytoestogens are a group of lipophillic plant compounds that can have estrogenic effects in animals; both tumorigenic and anti-tumorigenic effects have been reported. Prolactin-secreting adenomas are the most prevalent form of pituitary tumors in humans and have been linked to estrogen exposures. We examined the proliferative effects of phytoestrogens on a rat pituitary tumor cell line, GH3/B6/F10, originally subcloned from GH3 cells based on its ability to express high levels of the membrane estrogen receptor-alpha.
methodsWe measured the proliferative effects of these phytoestrogens using crystal violet staining, the activation of several mitogen-activated protein kinases (MAPKs) and their downstream targets via a quantitative plate immunoassay, and caspase enzymatic activities.
resultsFour phytoestrogens (coumestrol, daidzein, genistein, and trans-resveratrol) were studied over wide concentration ranges. Except trans-resveratrol, all phytoestrogens increased GH3/B6/F10 cell proliferation at some concentration relevant to dietary levels. All four phytoestrogens attenuated the proliferative effects of estradiol when administered simultaneously. All phytoestrogens elicited MAPK and downstream target activations, but with time course patterns that often differed from that of estradiol and each other. Using selective antagonists, we determined that MAPKs play a role in the ability of these phytoestrogens to elicit these responses. In addition, except for trans-resveratrol, a serum removal-induced extrinsic apoptotic pathway was blocked by these phytoestrogens.
conclusionPhytoestrogens can block physiological estrogen-induced tumor cell growth in vitro and can also stimulate growth at high dietary concentrations in the absence of endogenous estrogens; these actions are correlated with slightly different signaling response patterns. Consumption of these compounds should be considered in strategies to control endocrine tumor cell growth, such as in the pituitary.
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