ArticleRetrovirology2009
Visualizing fusion of pseudotyped HIV-1 particles in real time by live cell microscopy.
Article in Retrovirology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Tracking the protein conformational motions driving HIV-1 membrane fusion.Scientific reports · 2026Article
- Article
- Single-Virus Tracking: From Imaging Methodologies to Virological Applications.Chemical reviews · 2020Review
- Intact Viral Particle Counts Measured by Flow Virometry Provide Insight into the Infectivity and Genome Packaging Efficiency of Moloney Murine Leukemia Virus.Journal of virology · 2020Article
- Modeling Viral Spread.Annual review of virology · 2016Review
- Visualization of Content Release from Cell Surface-Attached Single HIV-1 Particles Carrying an Extra-Viral Fluorescent pH-Sensor.PloS one · 2016Article
- Investigating the role of F-actin in human immunodeficiency virus assembly by live-cell microscopy.Journal of virology · 2014Article
- In vitro analysis of human immunodeficiency virus particle dissociation: gag proteolytic processing influences dissociation kinetics.PloS one · 2014Article
- Correlative microscopy for 3D structural analysis of dynamic interactions.Journal of visualized experiments : JoVE · 2013Article
- Tracking image correlation: combining single-particle tracking and image correlation.Biophysical journal · 2013Article
- Anionic lipids are required for vesicular stomatitis virus G protein-mediated single particle fusion with supported lipid bilayers.The Journal of biological chemistry · 2013Article
- Live cell imaging of viral entry.Current opinion in virology · 2013Review
- Differential pH-dependent cellular uptake pathways among foamy viruses elucidated using dual-colored fluorescent particles.Retrovirology · 2012Article
- Cholesterol-rich microdomains as docking platforms for respiratory syncytial virus in normal human bronchial epithelial cells.Journal of virology · 2012Article
- Direct visualization of HIV-1 with correlative live-cell microscopy and cryo-electron tomography.Structure (London, England : 1993) · 2011Article
- Single-particle tracking as a quantitative microscopy-based approach to unravel cell entry mechanisms of viruses and pharmaceutical nanoparticles.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Imaging single retrovirus entry through alternative receptor isoforms and intermediates of virus-endosome fusion.PLoS pathogens · 2011Article
- A SNAP-tagged derivative of HIV-1--a versatile tool to study virus-cell interactions.PloS one · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMost retroviruses enter their host cells by fusing the viral envelope with the plasma membrane. Although the protein machinery promoting fusion has been characterized extensively, the dynamics of the process are largely unknown.
resultsWe generated human immunodeficiency virus-1 (HIV-1) particles pseudotyped with the envelope (Env) protein of ecotropic murine leukemia virus eMLV to study retrovirus entry at the plasma membrane using live-cell microscopy. This Env protein mediates highly efficient pH independent fusion at the cell surface and can be functionally tagged with a fluorescent protein. To detect fusion events, double labeled particles carrying one fluorophor in Env and the other in the matrix (MA) domain of Gag were generated and characterized. Fusion events were defined as loss of Env signal after virus-cell contact. Single particle tracking of >20,000 individual traces in two color channels recorded 28 events of color separation, where particles lost the Env protein, with the MA layer remaining stable at least for a short period. Fourty-five events were detected where both colors were lost simultaneously. Importantly, the first type of event was never observed when particles were pseudotyped with a non-fusogenic Env.
conclusionThese results reveal rapid retroviral fusion at the plasma membrane and permit studies of the immediate post-fusion events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.