ArticleJournal of the American Chemical Society2009
Impact of nonnatural amino acid mutagenesis on the in vivo function and binding modes of a transcriptional activator.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Noncanonical Amino Acids in Biocatalysis.Chemical reviews · 2024Review
- Reversible Dual-Covalent Molecular Locking of the 14-3-3/ERRγ Protein-Protein Interaction as a Molecular Glue Drug Discovery Approach.Journal of the American Chemical Society · 2023Article
- A Facile Platform to EngineerACS central science · 2022Article
- Bifunctional Non-Canonical Amino Acids: Combining Photo-Crosslinking with Click Chemistry.Biomolecules · 2020Review
- Electron-deficient p-benzoyl-l-phenylalanine derivatives increase covalent chemical capture yields for protein-protein interactions.Protein science : a publication of the Protein Society · 2019Article
- Genetically encoded photochemical covalent crosslinking within the Hcp-1 self-assembling bacterial secretion machinery.Amino acids · 2018Article
- Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions.Nature communications · 2017Article
- Engineering the Genetic Code in Cells and Animals: Biological Considerations and Impacts.Accounts of chemical research · 2017Review
- HDAC8 Substrates Identified by Genetically Encoded Active Site Photocrosslinking.Journal of the American Chemical Society · 2017Article
- Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry.Analytical chemistry · 2017Article
- From Fuzzy to Function: The New Frontier of Protein-Protein Interactions.Accounts of chemical research · 2017Article
- Discovery of Enzymatic Targets of Transcriptional Activators via in Vivo Covalent Chemical Capture.Journal of the American Chemical Society · 2016Article
- TRIC: Capturing the direct cellular targets of promoter-bound transcriptional activators.Protein science : a publication of the Protein Society · 2016Article
- Incorporation of Non-Canonical Amino Acids.Advances in experimental medicine and biology · 2015Review
- Direct and Propagated Effects of Small Molecules on Protein-Protein Interaction Networks.Frontiers in bioengineering and biotechnology · 2015Review
- Architecture of the TIM23 inner mitochondrial translocon and interactions with the matrix import motor.The Journal of biological chemistry · 2014Article
- Sequence context and crosslinking mechanism affect the efficiency of in vivo capture of a protein-protein interaction.Biopolymers · 2014Article
- Genetic code expansion as a tool to study regulatory processes of transcription.Frontiers in chemistry · 2014Review
- An in vivo photo-cross-linking approach reveals a homodimerization domain of Aha1 in S. cerevisiae.PloS one · 2014Article
- Sekikaic acid and lobaric acid target a dynamic interface of the coactivator CBP/p300.Angewandte Chemie (International ed. in English) · 2012Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Protein-protein interactions play an essential role in cellular function, and methods to discover and characterize them in their native context are of paramount importance for gaining a deeper understanding of biological networks. In this study, an enhanced nonsense suppression system was utilized to incorporate the nonnatural amino acid p-benzoyl-L-phenylalanine (pBpa) throughout the transcriptional activation domain of the prototypical eukaryotic transcriptional activator Gal4 in vivo (S. cerevisiae). Functional studies of the pBpa-containing Gal4 mutants suggest that this essential binding interface of Gal4 is minimally impacted by these substitutions, with both transcriptional activity and sensitivity to growth conditions maintained. Further supporting this are in vivo cross-linking studies, including the detection of a key binding partner of Gal4, the inhibitor protein Gal80. Cross-linking with a range of pBpa-containing mutants revealed a Gal4 x Gal80 binding interface that extends beyond that previously predicted by conventional strategies. Thus, this approach can be broadened to the discovery of novel binding partners of transcription factors, information that will be critical for the development of therapeutically useful small molecule modulators of these protein-protein interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.