ArticleMethods in molecular biology (Clifton, N.J.)2009
In vivo assay for tumor cell invasion.
Article in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Signatures of breast cancer metastasis at a glance.Journal of cell science · 2016Review
- Autocrine HBEGF expression promotes breast cancer intravasation, metastasis and macrophage-independent invasion in vivo.Oncogene · 2014Article
- Guiding intracortical brain tumour cells to an extracortical cytotoxic hydrogel using aligned polymeric nanofibres.Nature materials · 2014Article
- Mycoplasma hyorhinis activates the NLRP3 inflammasome and promotes migration and invasion of gastric cancer cells.PloS one · 2013Article
- Selective gene-expression profiling of migratory tumor cells in vivo predicts clinical outcome in breast cancer patients.Breast cancer research : BCR · 2012Article
- Phosphoinositide 3-kinase signaling is critical for ErbB3-driven breast cancer cell motility and metastasis.Oncogene · 2012Article
- Contribution of CXCL12 secretion to invasion of breast cancer cells.Breast cancer research : BCR · 2012Article
- Apoptosis inhibitor ARC promotes breast tumorigenesis, metastasis, and chemoresistance.Cancer research · 2011Article
- High-resolution multiphoton imaging of tumors in vivo.Cold Spring Harbor protocols · 2011Article
- In vivo invasion of head and neck squamous cell carcinoma cells does not require macrophages.The American journal of pathology · 2011Article
- Evaluation of tumor formation of three bladder cancer cell lines in nude mice.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2011Article
- Opposing roles of CXCR4 and CXCR7 in breast cancer metastasis.Breast cancer research : BCR · 2011Article
- Tumor-stroma: In vivo assays and intravital imaging to study cell migration and metastasis.Drug discovery today. Disease models · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We describe an in vivo invasion assay that enables the collection of invasive cells from the primary tumor. In addition to determination of the endogenous, unstimulated invasive properties of cells in vivo, the assay can take advantage of the chemotactic properties of cancer cells. Microneedles are filled with a mixture of extracellular matrix components such as Matrigel with or without a chemoattractant such as EGF, and then introduced into the primary tumor of a rat or mouse that is generated either by orthotopic injection of carcinoma cell lines or by a transgene such as polyoma Middle T. Over the course of 4 h the invasive cell population enters the needles while the animal is kept under anesthesia. At the end of the collection time, the invasive cells are extruded from the microneedles and can be analyzed in terms of the number and type of cells that invade in response to defined stimuli. By including pharmacological inhibitors in the needle, signaling pathways contributing to in vivo invasion can also be identified. This assay leads to a better understanding of the cell types and signaling involved in the tumor microenvironment, and has the potential to be applied to a variety of in vivo models.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.