ArticleThe Journal of biological chemistry2009
Biochemical characterization of two wheat phosphoethanolamine N-methyltransferase isoforms with different sensitivities to inhibition by phosphatidic acid.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- A Comprehensive Functional Analysis ofPlants (Basel, Switzerland) · 2025Article
- Drought and heat stress mediated activation of lipid signaling in plants: a critical review.Frontiers in plant science · 2023Review
- NMT1 and NMT3Plant physiology · 2018Article
- Cloning and Functional Analysis of Phosphoethanolamine Methyltransferase Promoter from Maize (Zea mays L.).International journal of molecular sciences · 2018Article
- Conformational changes in the di-domain structure ofThe Journal of biological chemistry · 2017Article
- C1 Metabolism Inhibition and Nitrogen Deprivation Trigger Triacylglycerol Accumulation inFrontiers in plant science · 2016Article
- Role of aminoalcoholphosphotransferases 1 and 2 in phospholipid homeostasis in Arabidopsis.The Plant cell · 2015Article
- Lipid biosynthesis and protein concentration respond uniquely to phosphate supply during leaf development in highly phosphorus-efficient Hakea prostrata.Plant physiology · 2014Article
- The intestinal microbiome of fish under starvation.BMC genomics · 2014Article
- Phosphatidic acid, a versatile water-stress signal in roots.Frontiers in plant science · 2013Review
- Phosphatidic acid binds to cytosolic glyceraldehyde-3-phosphate dehydrogenase and promotes its cleavage in Arabidopsis.The Journal of biological chemistry · 2013Article
- Acclimation responses of Arabidopsis thaliana to sustained phosphite treatments.Journal of experimental botany · 2013Article
- Structure and reaction mechanism of phosphoethanolamine methyltransferase from the malaria parasite Plasmodium falciparum: an antiparasitic drug target.The Journal of biological chemistry · 2012Article
- Phosphoethanolamine methyltransferases in phosphocholine biosynthesis: functions and potential for antiparasite therapy.FEMS microbiology reviews · 2011Review
- Identification of phosphomethylethanolamine N-methyltransferase from Arabidopsis and its role in choline and phospholipid metabolism.The Journal of biological chemistry · 2010Article
- Phosphoenolpyruvate carboxylase from C4 leaves is selectively targeted for inhibition by anionic phospholipids.Plant physiology · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants the triple methylation of phosphoethanolamine to phosphocholine catalyzed by phosphoethanolamine N-methyltransferase (PEAMT) is considered a rate-limiting step in the de novo synthesis of phosphatidylcholine. Besides being a major membrane phospholipid, phosphatidylcholine can be hydrolyzed into choline and phosphatidic acid. Phosphatidic acid is widely recognized as a second messenger in stress signaling, and choline can be oxidized within the chloroplast to yield the putative osmoprotectant glycine betaine. Here we describe the cloning and biochemical characterization of a second wheat PEAMT isoform that has a four times higher specific activity than the previously described WPEAMT/TaPEAMT1 enzyme and is less sensitive to product inhibition by S-adenosyl homocysteine, but more sensitive to inhibition by phosphocholine. Both enzymes follow a sequential random Bi Bi mechanism and show mixed-type product inhibition patterns with partial inhibition for TaPEAMT1 and a strong non-competitive component for TaPEAMT2. An induction of TaPEAMT protein expression and activity is observed after cold exposure, ahead of an increase in gene expression. Our results demonstrate direct repression of in vitro enzymatic activities by phosphatidic acid for both enzymes, with TaPEAMT1 being more sensitive than TaPEAMT2 in the physiological concentration range. Other lipid ligands identified in protein-lipid overlays are phosphoinositide mono- as well as some di-phosphates and cardiolipin. These results provide new insights into the complex regulatory circuits of phospholipid biosynthesis in plants and underline the importance of head group biosynthesis in adaptive stress responses.
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