ArticleMicrobiology (Reading, England)2010
The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae.
Article in Microbiology (Reading, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 58 citations in OpenAlex.
- Article
- Review
- Immunization with recombinantExperimental biology and medicine (Maywood, N.J.) · 2024Article
- The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute toMicroorganisms · 2023Article
- Isolation and Genetic Characterization ofMicroorganisms · 2022Article
- PplD is a de-N-acetylase of the cell wall linkage unit of streptococcal rhamnopolysaccharides.Nature communications · 2022Article
- Streptococcal Infections in Marine Mammals.Microorganisms · 2021Review
- Bacterial Strategies to Preserve Cell Wall Integrity Against Environmental Threats.Frontiers in microbiology · 2018Review
- From bacterial killing to immune modulation: Recent insights into the functions of lysozyme.PLoS pathogens · 2017Review
- Identification of some main Streptococcus iniae associated proteins: relationship.Veterinary research communications · 2017Article
- Article
- High quality draft genomic sequence of Flavobacterium enshiense DK69(T) and comparison among Flavobacterium genomes.Standards in genomic sciences · 2015Article
- Listeria monocytogenes is resistant to lysozyme through the regulation, not the acquisition, of cell wall-modifying enzymes.Journal of bacteriology · 2014Article
- Mycobacterium tuberculosis Rv1096 protein: gene cloning, protein expression, and peptidoglycan deacetylase activity.BMC microbiology · 2014Article
- Clostridium difficile extracytoplasmic function σ factor σV regulates lysozyme resistance and is necessary for pathogenesis in the hamster model of infection.Infection and immunity · 2014Article
- O-linked glycosylation sites profiling in Mycobacterium tuberculosis culture filtrate proteins.Journal of proteomics · 2014Article
- Article
- Streptococcus iniae SF1: complete genome sequence, proteomic profile, and immunoprotective antigens.PloS one · 2014Article
- Innate immune response to Streptococcus iniae infection in zebrafish larvae.Infection and immunity · 2013Article
- Single-cell sequencing provides clues about the host interactions of segmented filamentous bacteria (SFB).Genome research · 2012Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The aquatic zoonotic pathogen Streptococcus iniae represents a threat to the worldwide aquaculture industry and poses a risk to humans who handle raw fish. Because little is known about the mechanisms of S. iniae pathogenesis or virulence factors, we established a high-throughput system combining whole-genome pyrosequencing and transposon mutagenesis that allowed us to identify virulence proteins, including Pdi, the polysaccharide deacetylase of S. iniae, that we describe here. Using bioinformatics tools, we identified a highly conserved signature motif in Pdi that is also conserved in the peptidoglycan deacetylase PgdA protein family. A Deltapdi mutant was attenuated for virulence in the hybrid striped bass model and for survival in whole fish blood. Moreover, Pdi was found to promote bacterial resistance to lysozyme killing and the ability to adhere to and invade epithelial cells. On the other hand, there was no difference in the autolytic potential, resistance to oxidative killing or resistance to cationic antimicrobial peptides between S. iniae wild-type and Deltapdi. In conclusion, we have demonstrated that pdi is involved in S. iniae adherence and invasion, lysozyme resistance and survival in fish blood, and have shown that pdi plays a role in the pathogenesis of S. iniae. Identification of Pdi and other S. iniae virulence proteins is a necessary initial step towards the development of appropriate preventive and therapeutic measures against diseases and economic losses caused by this pathogen.
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