ArticleMicrobiology (Reading, England)2009
Influence of type IV pilus retraction on the architecture of the Neisseria gonorrhoeae-infected cell cortex.
Article in Microbiology (Reading, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Type IV pilus retraction is required formBio · 2024Article
- Bacterium of one thousand and one variants: genetic diversity ofMicrobial genomics · 2023Review
- An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Gonococcal invasion into epithelial cells depends on both cell polarity and ezrin.PLoS pathogens · 2021Article
- Neisseria gonorrhoeae evades autophagic killing by downregulating CD46-cyt1 and remodeling lysosomes.PLoS pathogens · 2019Article
- Article
- Environmental cues and symbiont microbe-associated molecular patterns function in concert to drive the daily remodelling of the crypt-cell brush border of the Euprymna scolopes light organ.Cellular microbiology · 2016Article
- Seminal Plasma Promotes Neisseria gonorrhoeae Aggregation and Biofilm Formation.Journal of bacteriology · 2016Article
- Host-Associated Genomic Features of the Novel Uncultured Intracellular Pathogen Ca. Ichthyocystis Revealed by Direct Sequencing of Epitheliocysts.Genome biology and evolution · 2016Article
- Article
- Expression of Opacity Proteins Interferes with the Transmigration of Neisseria gonorrhoeae across Polarized Epithelial Cells.PloS one · 2015Article
- Pili-Induced Clustering of N. gonorrhoeae Bacteria.PloS one · 2015Article
- Dynamics of brush border remodeling induced by enteropathogenic E. coli.Gut microbes · 2014Article
- Steered molecular dynamics simulations of a type IV pilus probe initial stages of a force-induced conformational transition.PLoS computational biology · 2013Article
- Construction and characterization of a derivative of Neisseria gonorrhoeae strain MS11 devoid of all opa genes.Journal of bacteriology · 2012Article
- Structure of the Vibrio cholerae Type IVb Pilus and stability comparison with the Neisseria gonorrhoeae type IVa pilus.Journal of molecular biology · 2012Article
- Techniques to measure pilus retraction forces.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Article
- N. elongata produces type IV pili that mediate interspecies gene transfer with N. gonorrhoeae.PloS one · 2011Article
- Vibrio cholerae El Tor TcpA crystal structure and mechanism for pilus-mediated microcolony formation.Molecular microbiology · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Early in infection, Neisseria gonorrhoeae can be observed to attach to the epithelial cell surface as microcolonies and induce dramatic changes to the host cell cortex. We tested the hypothesis that type IV pili (Tfp) retraction plays a role in the ultrastructure of both the host cell cortex and the bacterial microcolony. Using serial ultrathin sectioning, transmission electron microscopy and 3D reconstruction of serial 2D images, we have obtained what we believe to be the first 3D reconstructions of the N. gonorrhoeae-host cell interface, and determined the architecture of infected cell microvilli as well as the attached microcolony. Tfp connect both wild-type (wt) and Tfp retraction-deficient bacteria with each other, and with the host cell membrane. Tfp fibres and microvilli form a lattice in the wt microcolony and at its periphery. Wt microcolonies induce microvilli formation and increases of surface area, leading to an approximately ninefold increase in the surface area of the host cell membrane at the site of attachment. In contrast, Tfp retraction-deficient microcolonies do not affect these parameters. Wt microcolonies had a symmetrical, dome-shaped structure with a circular 'footprint', while Tfp retraction-deficient microcolonies were notably less symmetrical. These findings support a major role for Tfp retraction in microvilli and microcolony architecture. They are consistent with the biophysical attributes of Tfp and the effects of Tfp retraction on epithelial cell signalling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.