ArticleEuropean journal of epidemiology2009
Determinants of DNA yield and purity collected with buccal cell samples.
Article in European journal of epidemiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- PCR optimization for buccal swab-derived samples: overcoming sporadic inhibition with bovine serum albumin.BMC genomics · 2025Article
- Optimising recovery of DNA from minimally invasive sampling methods: Efficacy of buccal swabs, preservation strategy and DNA extraction approaches for amphibian studies.Ecology and evolution · 2024Article
- Article
- The significance of amelogenin loci from toothpicks as forensic evidence for sex determination.Journal of Taibah University Medical Sciences · 2023Article
- Comparison of five DNA extraction methods in three medicinal plants:Molecular biology research communications · 2023Article
- Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells.Frontiers in bioengineering and biotechnology · 2022Article
- Synthesis, characterization, DNA binding, topoisomerase inhibition, and apoptosis induction studies of a novel cobalt(III) complex with a thiosemicarbazone ligand.Journal of inorganic biochemistry · 2020Article
- A simple method of genomic DNA extraction from human samples for PCR-RFLP analysis.Journal of biomolecular techniques : JBT · 2013Article
- Predictors of mother and child DNA yields in buccal cell samples collected in pediatric cancer epidemiologic studies: a report from the Children's Oncology group.BMC genetics · 2013Article
- New studies, technology, and the progress of epidemiology.European journal of epidemiology · 2010Article
- The Shanghai Changfeng Study: a community-based prospective cohort study of chronic diseases among middle-aged and elderly: objectives and design.European journal of epidemiology · 2010Article
- The Generation R Study: design and cohort update 2010.European journal of epidemiology · 2010Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Buccal cells are an important source of DNA in epidemiological studies, but little is known about factors that influence amount and purity of DNA. We assessed these factors in a self-administered buccal cell collection procedure, obtained with three cotton swabs. In 2,451 patients DNA yield and in 1,033 patients DNA purity was assessed. Total DNA yield ranged from 0.08 to 1078.0 microg (median 54.3 microg; mean 82.2 microg +/- SD 92.6). The median UV 260:280 ratio, was 1.95. Samples from men yielded significantly more DNA (median 58.7 microg) than those from women (median 44.2 microg). Diuretic drug users had significantly lower purity (median 1.92) compared to other antihypertensive drug users (1.95). One technician obtained significantly lower DNA yields. Older age was associated with lower DNA purity. In conclusion, DNA yield from buccal swabs was higher in men and DNA purity was associated with age and the use of diuretics.
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