ArticleInternational journal of biomedical science : IJBS2008
The 'PREXCEL-Q Method' for qPCR.
Article in International journal of biomedical science : IJBS, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
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- Precision and efficacy of RNA-guided DNA integration in high-expressing muscle loci.Molecular therapy. Nucleic acids · 2024Article
- Investigation into how Odontotermes obesus maintains a predominantly Termitomyces monoculture in their fungus combs suggests a potential partnership with both fungi and bacteria.Communications biology · 2024Article
- An Improved Reference Gene for Detection of "Plants (Basel, Switzerland) · 2021Article
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- Dark-induced senescence of barley leaves involves activation of plastid transglutaminases.Amino acids · 2015Article
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- Human respiratory syncytial virus memphis 37 causes acute respiratory disease in perinatal lamb lung.BioResearch open access · 2014Article
- Sucrose stabilization of Respiratory Syncytial Virus (RSV) during nebulization and experimental infection.BMC research notes · 2014Article
- Increased concentration of iodide in airway secretions is associated with reduced respiratory syncytial virus disease severity.American journal of respiratory cell and molecular biology · 2014Article
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- Effects of formalin-inactivated respiratory syncytial virus (FI-RSV) in the perinatal lamb model of RSV.PloS one · 2013Article
- SNF1-related protein kinases type 2 are involved in plant responses to cadmium stress.Plant physiology · 2012Article
- Validation of kinetics similarity in qPCR.Nucleic acids research · 2012Review
- Respiratory syncytial virus infection is associated with an altered innate immunity and a heightened pro-inflammatory response in the lungs of preterm lambs.Respiratory research · 2011Article
- Lack of protein 4.1G causes altered expression and localization of the cell adhesion molecule nectin-like 4 in testis and can cause male infertility.Molecular and cellular biology · 2011Article
- Exogenous administration of vascular endothelial growth factor prior to human respiratory syncytial virus a2 infection reduces pulmonary pathology in neonatal lambs and alters epithelial innate immune responses.Experimental lung research · 2011Article
- Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues.BMC biotechnology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The purpose of this manuscript is to describe a reliable approach to quantitative real-time polymerase chain reaction (qPCR) assay development and project management, which is currently embodied in the Excel 2003-based software program named "PREXCEL-Q" (P-Q) (formerly known as "FocusField2-6Gallup-qPCRSet-upTool-001," "FF2-6-001 qPCR set-up tool" or "Iowa State University Research Foundation [ISURF] project #03407"). Since its inception from 1997-2007, the program has been well-received and requested around the world and was recently unveiled by its inventor at the 2008 Cambridge Healthtech Institute's Fourth Annual qPCR Conference in San Diego, CA. P-Q was subsequently mentioned in a review article by Stephen A. Bustin, an acknowledged leader in the qPCR field. Due to its success and growing popularity, and the fact that P-Q introduces a unique/defined approach to qPCR, a concise description of what the program is and what it does has become important. Sample-related inhibitory problems of the qPCR assay, sample concentration limitations, nuclease-treatment, reverse transcription (RT) and master mix formulations are all addressed by the program, enabling investigators to quickly, consistently and confidently design uninhibited, dynamically-sound, LOG-linear-amplification-capable, high-efficiency-of-amplification reactions for any type of qPCR. The current version of the program can handle an infinite number of samples.
Identifiers
19759920PMC2744046W2102888758What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.