ArticleNucleic acids research2009
The Staphylococcus aureus pSK41 plasmid-encoded ArtA protein is a master regulator of plasmid transmission genes and contains a RHH motif used in alternate DNA-binding modes.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Clinical Rel mutations that increase basal (p)ppGpp promote conjugal transfer of staphylococcal resistance plasmids.Microbiology (Reading, England) · 2026Article
- The Plasmidomic Landscape of Clinical Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- A clade of RHH proteins ubiquitous in Sulfolobales and their viruses regulates cell cycle progression.Nucleic acids research · 2023Article
- Regulation of Gram-Positive Conjugation.Frontiers in microbiology · 2019Review
- Staphylococcal Plasmids, Transposable and Integrative Elements.Microbiology spectrum · 2018Review
- Article
- Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection.Nature immunology · 2014Article
- Combinatorial targeting of ribbon-helix-helix artificial transcription factors to chimeric recognition sites.Nucleic acids research · 2012Article
- The transcription factor AmrZ utilizes multiple DNA binding modes to recognize activator and repressor sequences of Pseudomonas aeruginosa virulence genes.PLoS pathogens · 2012Article
- Segrosome assembly at the pliable parH centromere.Nucleic acids research · 2011Article
- Helicobacter pylori NikR protein exhibits distinct conformations when bound to different promoters.The Journal of biological chemistry · 2011Article
- Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100.Nucleic acids research · 2011Article
- In vivo recognition of the fecA3 target promoter by Helicobacter pylori NikR.Journal of bacteriology · 2011Article
- AmrZ beta-sheet residues are essential for DNA binding and transcriptional control of Pseudomonas aeruginosa virulence genes.Journal of bacteriology · 2010Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Plasmids harbored by Staphylococcus aureus are a major contributor to the spread of bacterial multi-drug resistance. Plasmid conjugation and partition are critical to the dissemination and inheritance of such plasmids. Here, we demonstrate that the ArtA protein encoded by the S. aureus multi-resistance plasmid pSK41 is a global transcriptional regulator of pSK41 genes, including those involved in conjugation and segregation. ArtA shows no sequence homology to any structurally characterized DNA-binding protein. To elucidate the mechanism by which it specifically recognizes its DNA site, we obtained the structure of ArtA bound to its cognate operator, ACATGACATG. The structure reveals that ArtA is representative of a new family of ribbon-helix-helix (RHH) DNA-binding proteins that contain extended, N-terminal basic motifs. Strikingly, unlike most well-studied RHH proteins ArtA binds its cognate operators as a dimer. However, we demonstrate that it is also able to recognize an atypical operator site by binding as a dimer-of-dimers and the extended N-terminal regions of ArtA were shown to be essential for this dimer-of-dimer binding mode. Thus, these data indicate that ArtA is a master regulator of genes critical for both horizontal and vertical transmission of pSK41 and that it can recognize DNA utilizing alternate binding modes.
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