ArticleJournal of virology2009
Identification of proteins from prunus persica that interact with peach latent mosaic viroid.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Viroids and Retrozymes: Plant Circular RNAs Capable of Autonomous Replication.Plants (Basel, Switzerland) · 2024Review
- Cellular roadmaps of viroid infection.Trends in microbiology · 2023Review
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- An Inside Look into Biological Miniatures: Molecular Mechanisms of Viroids.International journal of molecular sciences · 2021Review
- Integrated Proteo-Transcriptomic Analyses Reveal Insights into Regulation of Pollen Development Stages and Dynamics of Cellular Response to Apple Fruit Crinkle Viroid (AFCVd)-Infection inInternational journal of molecular sciences · 2020Article
- Ethylene is Involved in Symptom Development and Ribosomal Stress of Tomato Plants upon Citrus Exocortis Viroid Infection.Plants (Basel, Switzerland) · 2020Article
- Citrus exocortis viroid causes ribosomal stress in tomato plants.Nucleic acids research · 2019Article
- Article
- The Role of the Chloroplast in the Replication of Positive-Sense Single-Stranded Plant RNA Viruses.Frontiers in plant science · 2018Article
- Complete Genome Sequences of Peach Latent Mosaic Viroid from a Single Peach Cultivar.Genome announcements · 2015Article
- Prunus transcription factors: breeding perspectives.Frontiers in plant science · 2015Review
- What has been happening with viroids?Virus genes · 2014Review
- Synergistic roles of eukaryotic translation elongation factors 1Bγ and 1A in stimulation of tombusvirus minus-strand synthesis.PLoS pathogens · 2011Article
- Origin of hepatitis delta virus.Future microbiology · 2010Review
- The RNA strands of the plus and minus polarities of peach latent mosaic viroid fold into different structures.RNA (New York, N.Y.) · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peach latent mosaic viroid (PLMVd) is a small, single-stranded, circular RNA pathogen that infects Prunus persica trees. As with all other known viroids, the PLMVd genome does not encode any proteins. Consequently, it must interact with host cellular factors in order to ensure its life cycle. With the objective of identifying cellular proteins that interact with PLMVd, Northwestern hybridizations were performed using partially purified peach leaf extracts. Mass spectrometric analysis of the detected RNA-protein complexes led to the identification of six putative RNA-binding proteins. One of these was found to be elongation factor 1-alpha (eEF1A), and because of its known involvement in the replication and translation of various RNA viruses, further characterizations were performed. Initially, the existence of this interaction received support from an experiment that immunoprecipitated the eEF1A from a crude extract of infected peach leaves, coupled with reverse transcription-PCR detection of the PLMVd. Subsequently, eEF1A interaction with PLMVd strands of both polarities was confirmed in vitro by electrophoresis mobility shift assays, fluorescence spectroscopy, and the prediction of an altered PLMVd RNase mapping profile in the presence of the protein. The potential contribution of eEF1A to the molecular biology of PLMVd, including for viroid replication, is discussed.
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