ArticleBMC microbiology2009
High-level chromate resistance in Arthrobacter sp. strain FB24 requires previously uncharacterized accessory genes.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 87 citations in OpenAlex.
- Mixing of Two Microbial Consortia in the Search for Stimulating Chromium Depletion.International journal of microbiology · 2025Article
- Microbial Contributions to Heavy Metal Phytoremediation in Agricultural Soils: A Review.Microorganisms · 2024Review
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- Chromate tolerance and removal of bacterial strains isolated from uncontaminated and chromium-polluted environments.World journal of microbiology & biotechnology · 2019Article
- Biodegradation of persistent environmental pollutants by Arthrobacter sp.Environmental science and pollution research international · 2019Article
- Genomic and Transcriptomic Changes that Mediate Increased Platinum Resistance in Cupriavidus metallidurans.Genes · 2019Article
- Comparative genomics of 16PeerJ · 2019Article
- Chromate Resistance Mechanisms in Leucobacter chromiiresistens.Applied and environmental microbiology · 2018Article
- Article
- Draft genomic sequence of a chromate- and sulfate-reducing Alishewanella strain with the ability to bioremediate Cr and Cd contamination.Standards in genomic sciences · 2016Article
- Characterization and structure prediction of partial length protein sequences of pcoA, pcoR and chrB genes from heavy metal resistant bacteria from the Klip River, South Africa.International journal of molecular sciences · 2015Article
- Article
- The ins and outs of metal homeostasis by the root nodule actinobacterium Frankia.BMC genomics · 2014Article
- Global transcriptome analysis of hexavalent chromium stress responses in Staphylococcus aureus LZ-01.Ecotoxicology (London, England) · 2014Article
- Chromate alters root system architecture and activates expression of genes involved in iron homeostasis and signaling in Arabidopsis thaliana.Plant molecular biology · 2014Article
- Complete genome sequence of Arthrobacter sp. strain FB24.Standards in genomic sciences · 2013Article
- Article
- The Neurospora crassa chr-1 gene is up-regulated by chromate and its encoded CHR-1 protein causes chromate sensitivity and chromium accumulation.Current genetics · 2012Article
- Complete genome sequence and metabolic potential of the quinaldine-degrading bacterium Arthrobacter sp. Rue61a.BMC genomics · 2012Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe genome of Arthrobacter sp. strain FB24 contains a chromate resistance determinant (CRD), consisting of a cluster of 8 genes located on a 10.6 kb fragment of a 96 kb plasmid. The CRD includes chrA, which encodes a putative chromate efflux protein, and three genes with amino acid similarities to the amino and carboxy termini of ChrB, a putative regulatory protein. There are also three novel genes that have not been previously associated with chromate resistance in other bacteria; they encode an oxidoreductase (most similar to malate:quinone oxidoreductase), a functionally unknown protein with a WD40 repeat domain and a lipoprotein. To delineate the contribution of the CRD genes to the FB24 chromate [Cr(VI)] response, we evaluated the growth of mutant strains bearing regions of the CRD and transcript expression levels in response to Cr(VI) challenge.
resultsA chromate-sensitive mutant (strain D11) was generated by curing FB24 of its 96-kb plasmid. Elemental analysis indicated that chromate-exposed cells of strain D11 accumulated three times more chromium than strain FB24. Introduction of the CRD into strain D11 conferred chromate resistance comparable to wild-type levels, whereas deletion of specific regions of the CRD led to decreased resistance. Using real-time reverse transcriptase PCR, we show that expression of each gene within the CRD is specifically induced in response to chromate but not by lead, hydrogen peroxide or arsenate. Higher levels of chrA expression were achieved when the chrB orthologs and the WD40 repeat domain genes were present, suggesting their possible regulatory roles.
conclusionOur findings indicate that chromate resistance in Arthrobacter sp. strain FB24 is due to chromate efflux through the ChrA transport protein. More importantly, new genes have been identified as having significant roles in chromate resistance. Collectively, the functional predictions of these additional genes suggest the involvement of a signal transduction system in the regulation of chromate efflux and warrants further study.
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