ArticleMolecular biology reports2010
Validation of endogenous reference genes for insecticide-induced and developmental expression profiling of Liposcelis bostsrychophila (Psocoptera: Liposcelididae).
Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Screening Potential Reference Genes inGenes · 2021Article
- Article
- Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method inFrontiers in physiology · 2020Article
- Validation of reference genes for quantitative real-time PCR in tiger beetles across sexes, body parts, sexual maturity and immune challenge.Scientific reports · 2018Article
- Selection of Reference Genes for the Normalization of RT-qPCR Data in Gene Expression Studies in Insects: A Systematic Review.Frontiers in physiology · 2018Article
- Reference gene selection to determine differences in mitochondrial gene expressions in phosphine-susceptible and phosphine-resistant strains of Cryptolestes ferrugineus, using qRT-PCR.Scientific reports · 2017Article
- Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti.Scientific reports · 2017Article
- Exploring valid reference genes for quantitative real-time PCR analysis in Sesamia inferens (Lepidoptera: Noctuidae).PloS one · 2015Article
- Identification of suitable endogenous control genes for quantitative RT-PCR analysis of miRNA in bovine solid tissues.Molecular biology reports · 2014Article
- Selection and validation of reference genes for functional studies in the Calliphoridae family.Journal of insect science (Online) · 2014Article
- Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii.Journal of insect science (Online) · 2014Article
- Article
- Validation of reference genes for expression analysis by quantitative real-time PCR in Leptinotarsa decemlineata (Say).BMC research notes · 2013Article
- Exploring valid reference genes for quantitative real-time PCR analysis in Plutella xylostella (Lepidoptera: Plutellidae).International journal of biological sciences · 2013Article
- Molecular characterization and alternative splicing of a sodium channel and DSC1 ortholog genes in Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae).International journal of biological sciences · 2013Article
- Reference gene selection for qRT-PCR analysis in the sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae).PloS one · 2013Article
- Validation of reference genes for gene expression studies in Aphis glycines (Hemiptera: Aphididae).Journal of economic entomology · 2012Article
- Molecular characterization of three heat shock protein 70 genes and their expression profiles under thermal stress in the citrus red mite.Molecular biology reports · 2012Article
- Validation of reference genes for expression analysis in the salivary gland and the intestine of Rhodnius prolixus (Hemiptera, Reduviidae) under different experimental conditions by quantitative real-time PCR.BMC research notes · 2012Article
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Four housekeeping genes named Lbbeta-Actin1, Lbbeta-Actin2, Lbalpha-Tubulin, and LbGapdh were cloned from Liposcelis bostrychophila using the combined techniques of reverse transcriptase-PCR (RT-PCR) with rapid amplification of cDNA ends (RACE). The GenBank accession numbers were FJ196622, FJ447483, FJ595242, and FJ595241, respectively. The full-length cDNA of Lbbeta-Actin1 was a 1,772 bp sequence with an open reading frame (ORF) encoded 376 amino acids, while Lbbeta-Actin2 was 1,350 bp in length containing an ORF encoded 376 amino acids. Furthermore, the 1,565 bp cDNA of Lbalpha-Tubulin had an ORF of 1,350 bp encoding 450 amino acids. And LbGapdh possessed an ORF of 333 amino acids. Sequences analysis and phylogenetic trees generated from the nucleotide sequences of their coding regions revealed a relationship that was closer to other insects than to mammals. The four genes together with 18S rRNA were quantified for transcription stability in L. bostrychophila, and the geNorm software ranked from the most to least were Lbbeta-Actin1 > LbGapdh > Lbalpha-Tubulin > Lbbeta-Actin2 > Lb18S rRNA for deltamethrin induction, while ranked Lbbeta-Actin1 > Lbalpha-Tubulin > Lbbeta-Actin2 > LbGapdh > Lb18S rRNA for the different developmental stages.
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