ArticleMolecular and cellular biochemistry2010
The regulation of AMPK signaling in a natural state of profound metabolic rate depression.
Article in Molecular and cellular biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Vardenafil Alleviates Doxorubicin-Induced Cardiotoxicity Associated with Restoration of the AMPK/SIRT1 Signaling Pathway.Biomedicines · 2026Article
- Epitranscriptomics as a Candidate Universal Modulator of Dormancy Transitions.Ecology and evolution · 2026Review
- NO modulation of AMP-activated protein kinase: A key player in regulating beef tenderness during post-mortem maturation.Food chemistry: X · 2025Article
- The Role of PKGIα and AMPK Signaling Interplay in the Regulation of Albumin Permeability in Cultured Rat Podocytes.International journal of molecular sciences · 2023Article
- Neuroprotective Effect of Apigenin on Depressive-Like Behavior: Mechanistic Approach.Neurochemical research · 2022Article
- Organ protection by SGLT2 inhibitors: role of metabolic energy and water conservation.Nature reviews. Nephrology · 2021Review
- Hydration biomarkers and copeptin: relationship with ad libitum energy intake, energy expenditure, and metabolic fuel selection.European journal of clinical nutrition · 2020Article
- Article
- Transcriptome analysis of the threatened snail Ellobium chinense reveals candidate genes for adaptation and identifies SSRs for conservation genetics.Genes & genomics · 2018Article
- High salt intake reprioritizes osmolyte and energy metabolism for body fluid conservation.The Journal of clinical investigation · 2017Article
- Purification and Characterization of Lactate Dehydrogenase in the Foot Muscle and Hepatopancreas of Otala lactea.The protein journal · 2016Article
- Role of AMP-activated protein kinase in metabolic depression in animals.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Review
- Article
- Reduction in neural performance following recovery from anoxic stress is mimicked by AMPK pathway activation.PloS one · 2014Article
- Metformin reverses multidrug resistance and epithelial-mesenchymal transition (EMT) via activating AMP-activated protein kinase (AMPK) in human breast cancer cells.Molecular and cellular biochemistry · 2014Article
- Regulation of a truncated isoform of AMP-activated protein kinase α (AMPKα) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2013Article
- AMP-activated protein kinase plays a role in initiating metabolic rate suppression in goldfish hepatocytes.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2011Article
- A soluble guanylate cyclase-dependent mechanism is involved in the regulation of net hepatic glucose uptake by nitric oxide in vivo.Diabetes · 2010Article
- Involvement of AMPK in alcohol dehydrogenase accentuated myocardial dysfunction following acute ethanol challenge in mice.PloS one · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In response to energy stress (and elevated AMP), the AMP-activated protein kinase (AMPK) coordinates the restoration of energy homeostasis. We determined that AMPK is activated in a model system (desert snail Otala lactea) during a physiological state of profound metabolic rate depression (estivation) in the absence of a rise in AMP. Kinetic characterization indicated a strong increase in AMPK activity and phosphorylation in estivation, consistent with an increase in P-Ser428 LKB, an established regulator of AMPK. Accordingly, approximately 2-fold increases in AMPKalpha1 protein and activity were observed with LKB1 immunoprecipitates from estivating snails. In vitro studies determined that AMPK in crude extracts was activated in the presence of cGMP and deactivated in conditions that permitted protein phosphatase type-2A (PP2A) activity. Furthermore, AMPKalpha1 protein and activity increased in PKG immunoprecipitates from estivating tissues, suggesting a novel role for PKG in the regulation of AMPK in vivo. We evaluated several downstream targets of AMPK. Acetyl-CoA carboxylase (ACC) activity was strongly inhibited in estivation, consistent with increased P-Ser79 content, and in vitro stimulation of AMPK negated citrate's ability to stimulate ACC aggregation. Analysis of other targets revealed a strong decrease in PPARgamma-coactivator 1alpha expression in both tissues, which was related to decreased gluconeogenic protein expression in hepatic tissue, but no changes in mitochondrial biogenesis markers in muscle. We concluded that AMPK activation in O. lactea aids in facilitating the suppression of anabolic pathways, without necessarily activating ATP-generating catabolism.
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