ArticleParasitology research2009
Multiplex PCR system for identifying the carnivore origins of faeces for an epidemiological study on Echinococcus multilocularis in Hokkaido, Japan.
Article in Parasitology research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Presence of red foxes and contamination withFood and waterborne parasitology · 2026Article
- Spatioseasonal Comparison of Fecal Resistome and Pathogenome of Raccoon Dogs in Korea.EcoHealth · 2026Article
- Brief report prevalence of Echinococcus species in wild foxes in parts of Qinghai Province, China.Veterinary research communications · 2023Article
- EmergingTransboundary and emerging diseases · 2023Article
- Article
- DNA-based detection of Leptospira wolffii, Giardia intestinalis and Toxoplasma gondii in environmental feces of wild animals in Korea.The Journal of veterinary medical science · 2021Article
- Soil contamination by Echinococcus multilocularis in rural and urban vegetable gardens in relation to fox, cat and dog faecal deposits.Parasite (Paris, France) · 2021Article
- First report ofInternational journal for parasitology. Parasites and wildlife · 2020Article
- Multiplex PCRs for the specific identification of marsupial and deer species from faecal samples as a basis for non-invasive epidemiological studies of parasites.Parasites & vectors · 2020Article
- A One Health systematic review of diagnostic tools forFood and waterborne parasitology · 2019Article
- Investigation ofFrontiers in veterinary science · 2018Article
- Development of LAMP assays for the molecular detection of taeniid infection in canine in Tibetan rural area.The Journal of veterinary medical science · 2017Article
- Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis.Applied and environmental microbiology · 2016Article
- Survey on helminths in the small intestine of wild foxes in Qinghai, China.The Journal of veterinary medical science · 2013Article
- Ulnar mononeuropathy associated with eosinophilia.Paediatrics and international child health · 2012Article
- A real-time multiplex-nested PCR system for coprological diagnosis of Echinococcus multilocularis and host species.Parasitology research · 2011Article
- A Pilot Study on Deworming Wild Foxes forIranian journal of parasitologyArticle
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multiplex PCR system was developed to identify the carnivore origins of faeces collected in Hokkaido, Japan, for epidemiological studies on Echinococcus multilocularis. Primers were designed against the D-loop region of mitochondrial DNA. Two separate primer mixtures (mix 1, specific forward primers to fox, raccoon dog and dog, and a universal reverse primer [prH]; and mix 2, specific forward primers to cat, raccoon and weasels and prH) were used so that the PCR products (160 bp, fox and cat; 240 bp, raccoon dog and raccoon; and 330 bp, dog and weasel) were distinguished by size. The multiplex PCR exhibited no cross-reactivity between carnivore species and did not amplify DNA from rodent prey. When 270 field-collected faeces were examined, 250 showed single PCR products belonging to specific target sizes, suggesting successful carnivore identification for 92.6% of samples. Taeniid eggs were detected in 11.1% of samples and coproantigen in 30.4%; whereas the prevalences of taeniid eggs and coproantigen were 12.9% and 34.0% in fox faeces, and 0% and 26.3% in cat faeces, respectively. These results suggest that the prevalence in different target animals can be evaluated individually and precisely using multiplex PCR system.
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