ArticleMolecular & cellular proteomics : MCP2009
Defining elastic fiber interactions by molecular fishing: an affinity purification and mass spectrometry approach.
Article in Molecular & cellular proteomics : MCP, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Genotype-phenotype correlations of marfan syndrome and related fibrillinopathies: Phenomenon and molecular relevance.Frontiers in genetics · 2022Review
- Article
- The role of fibrillin and microfibril binding proteins in elastin and elastic fibre assembly.Matrix biology : journal of the International Society for Matrix Biology · 2019Review
- Fibrillin microfibrils and elastic fibre proteins: Functional interactions and extracellular regulation of growth factors.Seminars in cell & developmental biology · 2019Review
- Recombinant Extracellular Matrix Protein Fragments Support Human Embryonic Stem Cell Chondrogenesis.Tissue engineering. Part A · 2018Article
- Structural and compositional diversity of fibrillin microfibrils in human tissues.The Journal of biological chemistry · 2018Article
- Screening of differentially expressed proteins in psoriasis vulgaris by two-dimensional gel electrophoresis and mass spectrometry.Experimental and therapeutic medicine · 2017Article
- Fell-Muir Lecture: Fibrillin microfibrils: structural tensometers of elastic tissues?International journal of experimental pathology · 2017Review
- ADAMTS-10 and -6 differentially regulate cell-cell junctions and focal adhesions.Scientific reports · 2016Article
- Progranulin as a candidate biomarker for therapeutic trial in patients with ALS and FTLD.Journal of neural transmission (Vienna, Austria : 1996) · 2016Article
- Comparison of TGFbR2 down-regulation in expanded HSCs on MBA/DBM scaffolds coated by UCB stromal cells.In vitro cellular & developmental biology. Animal · 2015Article
- Epithelial-mesenchymal status influences how cells deposit fibrillin microfibrils.Journal of cell science · 2014Article
- Defining the extracellular matrix using proteomics.International journal of experimental pathology · 2013Review
- Human protein reference database and human proteinpedia as discovery resources for molecular biotechnology.Molecular biotechnology · 2011Article
- MatrixDB, the extracellular matrix interaction database.Nucleic acids research · 2011Article
- Construction of a large extracellular protein interaction network and its resolution by spatiotemporal expression profiling.Molecular & cellular proteomics : MCP · 2010Article
- Glycomics expression analysis of sulfated glycosaminoglycans of human colorectal cancer tissues and non-neoplastic mucosa by electrospray ionization mass spectrometry.Einstein (Sao Paulo, Brazil)Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Deciphering interacting networks of the extracellular matrix is a major challenge. We describe an affinity purification and mass spectrometry strategy that has provided new insights into the molecular interactions of elastic fibers, essential extracellular assemblies that provide elastic recoil in dynamic tissues. Using cell culture models, we defined primary and secondary elastic fiber interaction networks by identifying molecular interactions with the elastic fiber molecules fibrillin-1, MAGP-1, fibulin-5, and lysyl oxidase. The sensitivity and validity of our method was confirmed by identification of known interactions with the bait proteins. Our study revealed novel extracellular protein interactions with elastic fiber molecules and delineated secondary interacting networks with fibronectin and heparan sulfate-associated molecules. This strategy is a novel approach to define the macromolecular interactions that sustain complex extracellular matrix assemblies and to gain insights into how they are integrated into their surrounding matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.