ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2010
Gelatin in situ zymography on fixed, paraffin-embedded tissue: zinc and ethanol fixation preserve enzyme activity.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
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- Gelatin Zymography Can Be Performed on Fixed Brain Tissue.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
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- GSK2593074A blocks progression of existing abdominal aortic dilation.JVS-vascular science · 2020Article
- Change in Proteolytic Profile in Heifers After Oligofructose Overload.Frontiers in veterinary science · 2020Article
- Dual role of iodine, silver, chlorhexidine and octenidine as antimicrobial and antiprotease agents.PloS one · 2019Article
- Insulin-like growth factor receptor signaling in breast tumor epithelium protects cells from endoplasmic reticulum stress and regulates the tumor microenvironment.Breast cancer research : BCR · 2018Article
- Methods of Measuring Enzyme Activity Ex Vivo and In Vivo.Annual review of analytical chemistry (Palo Alto, Calif.) · 2018Review
- Tissue-type plasminogen activator contributes to remodeling of the rat ductus arteriosus.PloS one · 2018Article
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6 authors.
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Abstract
In situ zymography is a method for the detection and localization of enzymatic activity in tissue sections. This method is used with frozen sections because routine fixation of tissue in neutral-buffered formalin inhibits enzyme activity. However, frozen sections present with poor tissue morphology, making precise localization of enzymatic activity difficult to determine. Ethanol- and zinc-buffered fixative (ZBF) are known to preserve both morphological and functional properties of the tissue well, but it has not previously been shown that these fixatives preserve enzyme activity. In the present study, we show that in situ zymography can be performed on ethanol- and ZBF-fixed paraffin-embedded tissue. Compared with snap-frozen tissue, ethanol- and ZBF-fixed tissue showed stronger signals and superior morphology, allowing for a much more precise detection of gelatinolytic activity. Gelatinolytic enzymes could also be extracted from both ethanol- and ZBF-fixed tissue. The yield, as analyzed by SDS-PAGE gelatin zymography and Western blotting, was influenced by the composition of the extraction buffer, but was generally lower than that obtained from unfixed tissue.
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