ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2009
Genomic, tissue expression, and protein characterization of pCLCA1, a putative modulator of cystic fibrosis in the pig.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Proteomic profiling of equine airway mucus reveals compositional changes in asthmatic phenotypes.Scientific reports · 2026Article
- Chloride channel accessory 1 gene deficiency causes selective loss of mucus production in a new pig model.American journal of physiology. Lung cellular and molecular physiology · 2022Article
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- Calcium-activated chloride channel regulator 1 (CLCA1): More than a regulator of chloride transport and mucus production.The World Allergy Organization journal · 2019Article
- Soluble mucus component CLCA1 modulates expression of leukotactic cytokines and BPIFA1 in murine alveolar macrophages but not in bone marrow-derived macrophages.Histochemistry and cell biology · 2018Article
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- Murine CLCA5 is uniquely expressed in distinct niches of airway epithelial cells.Histochemistry and cell biology · 2015Article
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- Naturally Occurring Deletion Mutants of the Pig-Specific, Intestinal Crypt Epithelial Cell Protein CLCA4b without Apparent Phenotype.PloS one · 2015Article
- Impaired autoproteolytic cleavage of mCLCA6, a murine integral membrane protein expressed in enterocytes, leads to cleavage at the plasma membrane instead of the endoplasmic reticulum.Molecules and cells · 2012Article
- The porcine chloride channel calcium-activated family member pCLCA4a mirrors lung expression of the human hCLCA4.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Article
- The murine goblet cell protein mCLCA3 is a zinc-dependent metalloprotease with autoproteolytic activity.Molecules and cells · 2011Article
- Tissue and cellular expression patterns of porcine CFTR: similarities to and differences from human CFTR.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- Murine mCLCA5 is expressed in granular layer keratinocytes of stratified epithelia.Histochemistry and cell biology · 2010Article
- THOC5/FMIP, an mRNA export TREX complex protein, is essential for hematopoietic primitive cell survival in vivo.BMC biology · 2010Article
- The Chloride Channel Regulator, Calcium-Activated-1 Is Expressed in Synoviocytes and Articular Chondrocytes in Health and Disease.The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies have identified members of the CLCA (chloride channels, calcium-activated) gene family as potential modulators of the cystic fibrosis (CF) phenotype, but differences between the human and murine CLCA genes and proteins may limit the use of murine CF models. Recently established pig models of CF are expected to mimic the human disease more closely than the available mouse models do. Here, we characterized the porcine CLCA gene locus, analyzed the expression pattern and protein processing of pCLCA1, and compared it to its human ortholog, hCLCA1. The porcine CLCA gene family is located on chromosome 4q25, with a broad synteny with the human and murine clca gene loci, except for a pig-specific gene duplication of pCLCA4. Using pCLCA1-specific antibodies, the protein was immunohistochemically localized in mucin-producing cells, including goblet cells and mucinous glands in the respiratory and alimentary tracts. Similar to hCLCA1, biochemical characterization of pCLCA1 identified a secreted soluble protein that could serve as an extracellular signaling molecule or functional constituent of the protective mucous layers. The results suggest that pCLCA1 shares essential characteristics of hCLCA1, supporting the pig model as a promising tool for studying the modulating role of pCLCA1 in the complex pathology of CF.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.