ArticlePlant physiology2009
Phosphate (Pi) starvation effect on the cytosolic Pi concentration and Pi exchanges across the tonoplast in plant cells: an in vivo 31P-nuclear magnetic resonance study using methylphosphonate as a Pi analog.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 131 citations in OpenAlex.
- Temporal Phosphorus Allocation and Resorption Plasticity Drive Interspecific Phosphorus-Use Efficiency in Rice-Rapeseed Rotations.Plant, cell & environment · 2026Article
- Systematic characterization of purple acid phosphatases provides targets for improving phosphorus use efficiency in cotton.Functional & integrative genomics · 2026Article
- Genome-wide systematic characterization of PHT gene family and its member involved in phosphate uptake in Orychophragmus violaceus.BMC genomics · 2025Article
- The functional mechanisms of phosphite and its applications in crop plants.Frontiers in plant science · 2025Review
- OsMYB58 Negatively Regulates Plant Growth and Development by Regulating Phosphate Homeostasis.International journal of molecular sciences · 2024Article
- Monitoring nutrients in plants with genetically encoded sensors: achievements and perspectives.Plant physiology · 2023Review
- Variability and limits of nitrogen and phosphorus resorption during foliar senescence.Plant communications · 2023Review
- Mechanisms for improving phosphorus utilization efficiency in plants.Annals of botany · 2022Review
- Phosphate-deprivation and damage signalling by extracellular ATP.Frontiers in plant science · 2022Article
- Intracellular phosphate sensing and regulation of phosphate transport systems in plants.Plant physiology · 2021Review
- Potential Networks of Nitrogen-Phosphorus-Potassium Channels and Transporters in Arabidopsis Roots at a Single Cell Resolution.Frontiers in plant science · 2021Article
- Genome-wide analysis of haloacid dehalogenase genes reveals their function in phosphate starvation responses in rice.PloS one · 2021Article
- Spatial Profiles of Phosphate in Roots Indicate Developmental Control of Uptake, Recycling, and Sequestration.Plant physiology · 2020Article
- Live Imaging of Phosphate Levels in Arabidopsis Root Cells Expressing a FRET-Based Phosphate Sensor.Plants (Basel, Switzerland) · 2020Article
- Purple acid phosphatase 10c encodes a major acid phosphatase that regulates plant growth under phosphate-deficient conditions in rice.Journal of experimental botany · 2020Article
- Biotechnological mechanism for improving plant remobilization of phosphorus during leaf senescence.Plant biotechnology journal · 2020Article
- An Update on Nitric Oxide Production and Role Under Phosphorus Scarcity in Plants.Frontiers in plant science · 2020Review
- Knockout ofFrontiers in plant science · 2020Article
- Parenchyma Abundance in Wood of Evergreen Trees Varies Independently of Nutrients.Frontiers in plant science · 2020Article
- Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence.Environmental science and pollution research international · 2020Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo (31)P-NMR analyses showed that the phosphate (Pi) concentration in the cytosol of sycamore (Acer pseudoplatanus) and Arabidopsis (Arabidopsis thaliana) cells was much lower than the cytoplasmic Pi concentrations usually considered (60-80 mum instead of >1 mm) and that it dropped very rapidly following the onset of Pi starvation. The Pi efflux from the vacuole was insufficient to compensate for the absence of external Pi supply, suggesting that the drop of cytosolic Pi might be the first endogenous signal triggering the Pi starvation rescue metabolism. Successive short sequences of Pi supply and deprivation showed that added Pi transiently accumulated in the cytosol, then in the stroma and matrix of organelles bounded by two membranes (plastids and mitochondria, respectively), and subsequently in the vacuole. The Pi analog methylphosphonate (MeP) was used to analyze Pi exchanges across the tonoplast. MeP incorporated into cells via the Pi carrier of the plasma membrane; it accumulated massively in the cytosol and prevented Pi efflux from the vacuole. This blocking of vacuolar Pi efflux was confirmed by in vitro assays with purified vacuoles. Subsequent incorporation of Pi into the cells triggered a massive transfer of MeP from the cytosol to the vacuole. Mechanisms for Pi exchanges across the tonoplast are discussed in the light of the low cytosolic Pi level, the cell response to Pi starvation, and the Pi/MeP interactive effects.
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