ArticleNucleic acids research2009
PiggyBac transgenic strategies in the developing chicken spinal cord.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Genome-Wide Landscape of Position Effect Variegation inJournal of fungi (Basel, Switzerland) · 2026Article
- Genome Editing in the Chicken: From PGC-Mediated Germline Transmission to Advanced Applications.International journal of molecular sciences · 2025Review
- N-cadherin facilitates trigeminal sensory neuron outgrowth and target tissue innervation.Development (Cambridge, England) · 2025Article
- Cell Fate of Retinal Progenitor Cells: In Ovo UbC-StarTrack Analysis.International journal of molecular sciences · 2022Article
- A piggyBac-mediated transgenesis system for the temporary expression of CRISPR/Cas9 in rice.Plant biotechnology journal · 2021Article
- Article
- Expression profiling of sexually dimorphic genes in the Japanese quail, Coturnix japonica.Scientific reports · 2020Article
- Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development.Biology open · 2020Article
- Temporal-specific roles of fragile X mental retardation protein in the development of the hindbrain auditory circuit.Development (Cambridge, England) · 2020Article
- Simplified platform for mosaic in vivo analysis of cellular maturation in the developing heart.Scientific reports · 2019Article
- In VivoInternational journal of molecular sciences · 2019Article
- The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonicalThe Journal of biological chemistry · 2018Article
- Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase.Nucleic acids research · 2018Article
- Applications of Gene Editing in Chickens: A New Era Is on the Horizon.Frontiers in genetics · 2018Review
- Proliferation and Survival of Embryonic Sympathetic Neuroblasts by MYCN and Activated ALK Signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Rapid and efficient gene delivery into the adult mouse brain via focal electroporation.Scientific reports · 2016Article
- A regulatory sequence from theMolecular vision · 2016Article
- RNA-Seq analysis of differential gene expression in electroporated chick embryonic spinal cord.Journal of visualized experiments : JoVE · 2014Article
- A 'tool box' for deciphering neuronal circuits in the developing chick spinal cord.Nucleic acids research · 2014Article
- A synthetic biology approach identifies the mammalian UPR RNA ligase RtcB.Molecular cell · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The chicken spinal cord is an excellent model for the study of early neural development in vertebrates. However, the lack of robust, stable and versatile transgenic methods has limited the usefulness of chick embryos for the study of later neurodevelopmental events. Here we describe a new transgenic approach utilizing the PiggyBac (PB) transposon to facilitate analysis of late-stage neural development such as axon targeting and synaptic connection in the chicken embryo. Using PB transgenic approaches we achieved temporal and spatial regulation of transgene expression and performed stable RNA interference (RNAi). With these new capabilities, we mapped axon projection patterns of V2b subset of spinal interneurons and visualized maturation of the neuromuscular junction (NMJ). Furthermore, PB-mediated RNAi in the chick recapitulated the phenotype of loss of agrin function in the mouse NMJ. The simplicity and versatility of PB-mediated transgenic strategies hold great promise for large-scale genetic analysis of neuronal connectivity in the chick.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.