ArticleThe Journal of biological chemistry2009
Elevation of glutamine level by selenophosphate synthetase 1 knockdown induces megamitochondrial formation in Drosophila cells.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- De novo missense variants in exon 9 of SEPHS1 cause a neurodevelopmental condition with developmental delay, poor growth, hypotonia, and dysmorphic features.American journal of human genetics · 2024Article
- Mitochondrial Differentiation during Spermatogenesis: Lessons fromInternational journal of molecular sciences · 2024Review
- A comparative analysis of fruit fly and human glutamate dehydrogenases inFrontiers in cell and developmental biology · 2023Article
- SEPHS1: Its evolution, function and roles in development and diseases.Archives of biochemistry and biophysics · 2022Article
- SPS1 deficiency-triggered PGRP-LC and Toll expression controls innate immunity in Drosophila S2 cells.Biology open · 2022Article
- Constitutive Oxidative Stress by SEPHS1 Deficiency Induces Endothelial Cell Dysfunction.International journal of molecular sciences · 2021Article
- Identification of Signaling Pathways for Early Embryonic Lethality and Developmental Retardation inInternational journal of molecular sciences · 2021Article
- A glutamine sensor that directly activates TORC1.Communications biology · 2021Article
- A Genetic Screen Using theG3 (Bethesda, Md.) · 2019Article
- Selenophosphate synthetase 1 (SPS1) is required for the development and selenium homeostasis of central nervous system in chicken (Gallus gallus).Oncotarget · 2017Article
- Selenophosphate synthetase 1 is an essential protein with roles in regulation of redox homoeostasis in mammals.The Biochemical journal · 2016Article
- Article
- Cell Proliferation and Motility Are Inhibited by G1 Phase Arrest in 15-kDa Selenoprotein-Deficient Chang Liver Cells.Molecules and cells · 2015Article
- Deficiency of the 15-kDa selenoprotein led to cytoskeleton remodeling and non-apoptotic membrane blebbing through a RhoA/ROCK pathway.Biochemical and biophysical research communications · 2015Article
- Selenoproteins: molecular pathways and physiological roles.Physiological reviews · 2014Review
- l(2)01810 is a novel type of glutamate transporter that is responsible for megamitochondrial formation.The Biochemical journal · 2011Article
- Drosophila selenophosphate synthetase 1 regulates vitamin B6 metabolism: prediction and confirmation.BMC genomics · 2011Article
- Biosynthesis of selenocysteine, the 21st amino acid in the genetic code, and a novel pathway for cysteine biosynthesis.Advances in nutrition (Bethesda, Md.) · 2011Review
- Human selenophosphate synthetase 1 has five splice variants with unique interactions, subcellular localizations and expression patterns.Biochemical and biophysical research communications · 2010Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
Although selenophosphate synthetase 1 (SPS1/SelD) is an essential gene in Drosophila, its function has not been determined. To elucidate its intracellular role, we targeted the removal of SPS1/SelD mRNA in Drosophila SL2 cells using RNA interference technology that led to the formation of vacuole-like globular structures. Surprisingly, these structures were identified as megamitochondria, and only depolarized mitochondria developed into megamitochondria. The mRNA levels of l(2)01810 and glutamine synthetase 1 (GS1) were increased by SPS1/SelD knockdown. Blocking the expression of GS1 and l(2)01810 completely inhibited the formation of megamitochondria induced by loss of SPS1/SelD activity and decreased the intracellular levels of glutamine to those of control cells suggesting that the elevated level of glutamine is responsible for megamitochondrial formation. Overexpression of GS1 and l(2)01810 had a synergistic effect on the induction of megamitochondrial formation and on the synthesis of glutamine suggesting that l(2)01810 is involved in glutamine synthesis presumably by activating GS1. Our results indicate that, in Drosophila, SPS1/SelD regulates the intracellular glutamine by inhibiting GS1 and l(2)01810 expression and that elevated levels of glutamine lead to a nutritional stress that provides a signal for megamitochondrial formation.
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Registered trials
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