ArticleInsect molecular biology2009
Stability and loss of a virus resistance phenotype over time in transgenic mosquitoes harbouring an antiviral effector gene.
Article in Insect molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Performance of two low-threshold population replacement gene drives in cage populations of the yellow fever mosquito, Aedes aegypti.PLoS genetics · 2025Article
- Article
- Large-scale reference-free analysis of flavivirus sequences in Aedes aegypti whole genome DNA sequencing data.Parasites & vectors · 2023Article
- Assessing single-locus CRISPR/Cas9-based gene drive variants in the mosquito Aedes aegypti via single-generation crosses and modeling.G3 (Bethesda, Md.) · 2022Article
- Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus.Scientific reports · 2021Article
- Review
- The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in TransgenicViruses · 2020Article
- Leaning Into the Bite: The piRNA Pathway as an Exemplar for the Genetic Engineering Need in Mosquitoes.Frontiers in cellular and infection microbiology · 2020Article
- Arbovirus-Mosquito Vector-Host Interactions and the Impact on Transmission and Disease Pathogenesis of Arboviruses.Frontiers in microbiology · 2019Review
- Blood meal-induced inhibition of vector-borne disease by transgenic microbiota.Nature communications · 2018Article
- Viral Determinants and Vector Competence of Zika Virus Transmission.Frontiers in microbiology · 2018Review
- Review
- Disruption of dengue virus transmission by mosquitoes.Current opinion in insect science · 2015Article
- Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes.Insects · 2015Review
- Flavivirus-mosquito interactions.Viruses · 2014Review
- Fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse Aedes aegypti strain.PLoS neglected tropical diseases · 2014Article
- Novel Genetic and Molecular Tools for the Investigation and Control of Dengue Virus Transmission by Mosquitoes.Current tropical medicine reports · 2014Article
- Heritable strategies for controlling insect vectors of disease.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Genetic control of Aedes mosquitoes.Pathogens and global health · 2013Review
- Exogenous gypsy insulator sequences modulate transgene expression in the malaria vector mosquito, Anopheles stephensi.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Transgenic Aedes aegypti were engineered to express a virus-derived, inverted repeat (IR) RNA in the mosquito midgut to trigger RNA interference (RNAi) and generate resistance to dengue virus type 2 (DENV2) in the vector. Here we characterize genotypic and phenotypic stabilities of one line, Carb77, between generations G(9) and G(17). The anti-DENV2 transgene was integrated at a single site within a noncoding region of the mosquito genome. The virus resistance phenotype was strong until G(13) and suppressed replication of different DENV2 genotypes. From G(14)-G(17) the resistance phenotype to DENV2 became weaker and eventually was lost. Although the sequence of the transgene was not mutated, expression of the IR effector RNA was not detected and the Carb77 G(17) mosquitoes lost their ability to silence the DENV2 genome.
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Registered trials
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