ArticlePloS one2009
The mitotic arrest deficient protein MAD2B interacts with the small GTPase RAN throughout the cell cycle.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Docking-Based Virtual Screening Identifies a Small-Molecule Inhibitor of the Translesion Synthesis REV7/REV3 Protein-Protein Interaction.ChemMedChem · 2026Article
- Probing hot spots of protein-protein interactions mediated by the safety-belt region of REV7.Structure (London, England : 1993) · 2024Article
- REV7: a small but mighty regulator of genome maintenance and cancer development.Frontiers in oncology · 2024Review
- REV7 in Cancer Biology and Management.Cancers · 2023Review
- MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair.Nature communications · 2021Article
- REV7: Jack of many trades.Trends in cell biology · 2021Review
- DNA polymerase ζ in DNA replication and repair.Nucleic acids research · 2019Review
- Mitotic Arrest-Deficient Protein 2B Overexpressed in Lung Cancer Promotes Proliferation, EMT, and Metastasis.Oncology research · 2019Article
- SHLD2/FAM35A co-operates with REV7 to coordinate DNA double-strand break repair pathway choice.The EMBO journal · 2018Article
- Rev7 dimerization is important for assembly and function of the Rev1/Polζ translesion synthesis complex.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells.The EMBO journal · 2018Article
- Dynamic feature of mitotic arrest deficient 2-like protein 2 (MAD2L2) and structural basis for its interaction with chromosome alignment-maintaining phosphoprotein (CAMP).The Journal of biological chemistry · 2017Article
- The multifaceted roles of the HORMA domain in cellular signaling.The Journal of cell biology · 2015Review
- Rev7/Mad2B plays a critical role in the assembly of a functional mitotic spindle.Cell cycle (Georgetown, Tex.) · 2015Article
- Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset.The Journal of cell biology · 2013Article
- A critical function of Mad2l2 in primordial germ cell development of mice.PLoS genetics · 2013Article
- Rev3, the catalytic subunit of Polζ, is required for maintaining fragile site stability in human cells.Nucleic acids research · 2013Article
- CAMP (C13orf8, ZNF828) is a novel regulator of kinetochore-microtubule attachment.The EMBO journal · 2011Article
- The mitotic arrest deficient protein MAD2B interacts with the clathrin light chain A during mitosis.PloS one · 2010Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPreviously, we identified the mitotic arrest deficient protein MAD2B (MAD2L2) as a bona fide interactor of the renal cell carcinoma (RCC)-associated protein PRCC. In addition, we found that fusion of PRCC with the transcription factor TFE3 in t(X;1)(p11;q21)-positive RCCs results in an impairment of this interaction and, concomitantly, an abrogation of cell cycle progression. Although MAD2B is thought to inhibit the anaphase promoting complex (APC) by binding to CDC20 and/or CDH1(FZR1), its exact role in cell cycle control still remains to be established. METHODOLOGY/PRINCIPAL
findingsUsing a yeast two-hybrid interaction trap we identified the small GTPase RAN, a well-known cell cycle regulator, as a novel MAD2B binding protein. Endogenous interaction was established in mammalian cells via co-localization and co-immunoprecipitation of the respective proteins. The interaction domain of RAN could be assigned to a C-terminal moiety of 60 amino acids, whereas MAD2B had to be present in its full-length conformation. The MAD2B-RAN interaction was found to persist throughout the cell cycle. During mitosis, co-localization at the spindle was observed. CONCLUSIONS/SIGNIFICANCE: The small GTPase RAN is a novel MAD2B binding protein. This novel protein-protein interaction may play a role in (i) the control over the spindle checkpoint during mitosis and (ii) the regulation of nucleocytoplasmic trafficking during interphase.
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