ArticleBiophysical journal2009
On the origin of kinesin limping.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- The mechanochemistry of the kinesin-2 KIF3AC heterodimer is related to strain-dependent kinetic properties of KIF3A and KIF3C.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Force Dependence of Velocity and Run Length of Kinesin-1, Kinesin-2 and Kinesin-5 Family Molecular Motors.Molecules (Basel, Switzerland) · 2019Article
- Kinesin rotates unidirectionally and generates torque while walking on microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Coiled-coils: The long and short of it.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Article
- The winch model can explain both coordinated and uncoordinated stepping of cytoplasmic dynein.Biophysical journal · 2014Article
- Motor proteins and molecular motors: how to operate machines at the nanoscale.Journal of physics. Condensed matter : an Institute of Physics journal · 2013Review
- From structure to function: mitochondrial morphology, motion and shaping in vascular smooth muscle.Journal of vascular research · 2013Review
- Functional asymmetry in kinesin and dynein dimers.Biology of the cell · 2013Review
- Productive cooperation among processive motors depends inversely on their mechanochemical efficiency.Biophysical journal · 2011Article
- Electrons, photons, and force: quantitative single-molecule measurements from physics to biology.ACS nano · 2011Review
- Regulation of a heterodimeric kinesin-2 through an unprocessive motor domain that is turned processive by its partner.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- FRET measurements of kinesin neck orientation reveal a structural basis for processivity and asymmetry.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Kinesin is a dimeric motor with twin catalytic heads joined to a common stalk. Kinesin molecules move processively along microtubules in a hand-over-hand walk, with the two heads advancing alternately. Recombinant kinesin constructs with short stalks have been found to "limp", i.e., exhibit alternation in the dwell times of successive steps. Limping behavior implies that the molecular rearrangements underlying even- and odd-numbered steps must differ, but the mechanism by which such rearrangements lead to limping remains unsolved. Here, we used an optical force clamp to measure individual, recombinant dimers and test candidate explanations for limping. Introducing a covalent cross-link into the stalk region near the heads had no effect on limping, ruling out possible stalk misregistration during coiled-coil formation as a cause. Limping was equally unaffected by mutations that produced 50-fold changes in stalk stiffness, ruling out models where limping arises from an asymmetry in torsional strain. However, limping was enhanced by perturbations that increased the vertical component of load on the motor, including increases in bead size or net load, and decreases in the stalk length. These results suggest that kinesin heads take different vertical trajectories during alternate steps, and that the rates for these motions are differentially sensitive to load.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.