ArticleGenome biology2009
Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae.
Article in Genome biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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109 citing papers in PubMed, 222 citations in OpenAlex.
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- Mitochondrial RNA Helicases: Key Players in the Regulation of Plant Organellar RNA Splicing and Gene Expression.International journal of molecular sciences · 2024Review
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- More than Just Bread and Wine: Using Yeast to Understand Inherited Cytochrome Oxidase Deficiencies in Humans.International journal of molecular sciences · 2024Review
- From beer to breadboards: yeast as a force for biological innovation.Genome biology · 2024Review
- Severe mitochondrial encephalomyopathy caused byFrontiers in genetics · 2024Article
- Loss of mitochondrial DNA is associated with reduced DNA content variability inmicroPublication biology · 2024Article
- Parallel laboratory evolution and rational debugging reveal genomic plasticity toCell genomics · 2023Article
- Prefoldin 2 contributes to mitochondrial morphology and function.BMC biology · 2023Article
- Yeast Lsm Pro-Apoptotic Mutants Show Defects in Autophagy.International journal of molecular sciences · 2023Article
- Mitochondrial networks through the lens of mathematics.Physical biology · 2023Review
- Global analysis of the yeast knockout phenome.Science advances · 2023Article
- Toxicity and assimilation of cellulosic copper nanoparticles require α-arrestins in S. cerevisiae.Metallomics : integrated biometal science · 2023Article
- Mapping mitonuclear epistasis using a novel recombinant yeast population.PLoS genetics · 2023Article
49 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe mitochondrial respiratory chain produces metabolic energy by oxidative phosphorylation. Biogenesis of the respiratory chain requires the coordinated expression of two genomes: the nuclear genome encoding the vast majority of mitochondrial proteins, and the mitochondrial genome encoding a handful of mitochondrial proteins. The understanding of the molecular processes contributing to respiratory chain assembly and maintenance requires the systematic identification and functional analysis of the genes involved.
resultsWe pursued a systematic, genome-wide approach to define the sets of genes required for respiratory activity and maintenance and expression of the mitochondrial genome in yeast. By comparative gene deletion analysis we found an unexpected phenotypic plasticity among respiratory-deficient mutants, and we identified ten previously uncharacterized genes essential for respiratory growth (RRG1 through RRG10). Systematic functional analysis of 319 respiratory-deficient mutants revealed 16 genes essential for maintenance of the mitochondrial genome, 88 genes required for mitochondrial protein translation, and 10 genes required for expression of specific mitochondrial gene products. A group of mutants acquiring irreversible damage compromising respiratory capacity includes strains defective in assembly of the cytochrome c oxidase that were found to be particularly sensitive to aging.
conclusionsThese data advance the understanding of the molecular processes contributing to maintenance of the mitochondrial genome, mitochondrial protein translation, and assembly of the respiratory chain. They revealed a number of previously uncharacterized components, and provide a comprehensive picture of the molecular processes required for respiratory activity in a simple eukaryotic cell.
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