ArticleToxicology mechanisms and methods2009
Developmental toxicity and brain aromatase induction by high genistein concentrations in zebrafish embryos.
Article in Toxicology mechanisms and methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 47 citations in OpenAlex.
- Environmental estrogens and animal reproductive health: mechanisms, biomarkers, and intervention approaches.Journal of animal science and biotechnology · 2026Review
- Cholesterol modified defense peptide as an EMP2-siRNA delivery system for synergistic immunogene therapy against breast cancer.Materials today. Bio · 2025Article
- Potential Neuroprotective Role of Calretinin-N18 and Calbindin-D28k in the Retina of Adult Zebrafish Exposed to Different Wavelength Lights.International journal of molecular sciences · 2023Article
- Article
- Embryonic exposure to genistein induces anxiolytic and antisocial behavior in zebrafish: persistent effects until the adult stage.Environmental science and pollution research international · 2022Article
- Genistein: A Review on its Anti-Inflammatory Properties.Frontiers in pharmacology · 2022Review
- Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (International journal of molecular sciences · 2020Article
- Effects of Phytoestrogens on the Developing Brain, Gut Microbiota, and Risk for Neurobehavioral Disorders.Frontiers in nutrition · 2019Review
- The developmental effects of isoflavone aglycone administration on early chick embryos.Interdisciplinary toxicology · 2018Article
- Transcriptomic and phenotypic profiling in developing zebrafish exposed to thyroid hormone receptor agonists.Reproductive toxicology (Elmsford, N.Y.) · 2018Article
- Effects of the isoflavone genistein in early life stages of the Senegalese sole, Solea senegalensis: role of the Survivin and proliferation versus apoptosis pathways.BMC veterinary research · 2018Article
- Comparative Developmental Toxicity of Flavonoids Using an Integrative Zebrafish System.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Article
- Genistein and daidzein repress adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells via Wnt/β-catenin signalling or lipolysis.Cell proliferation · 2010Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genistein is a phytoestrogen found at a high level in soybeans. In vitro and in vivo studies showed that high concentrations of genistein caused toxic effects. This study was designed to test the feasibility of zebrafish embryos for evaluating developmental toxicity and estrogenic potential of high genistein concentrations. The zebrafish embryos at 24 h post-fertilization were exposed to genistein (1 x 10(-4) M, 0.5 x 10(-4) M, 0.25 x 10(-4) M) or vehicle (ethanol, 0.1%) for 60 h. Genistein-treated embryos showed decreased heart rates, retarded hatching times, decreased body length, and increased mortality in a dose-dependent manner. After 0.25 x 10(-4) M genistein treatment, malformations of survived embryos such as pericardial edema, yolk sac edema, and spinal kyphosis were also observed. TUNEL assay results showed apoptotic DNA fragments in brain. This study also confirmed the estrogenic potential of genistein by EGFP expression in the brain of the mosaic reporter zebrafish embryos. This study first demonstrated that high concentrations of genistein caused a teratogenic effect on zebrafish embryos and confirmed the estrogenic potential of genistein in mosaic reporter zebrafish embryos.
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