ArticlePloS one2009
High-density microwell chip for culture and analysis of stem cells.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 66 citations in OpenAlex.
- A universal platform for selection and high-resolution phenotypic screening of bacterial mutants using the nanowell slide.Lab on a chip · 2018Article
- A novel dual-well array chip for efficiently trapping single-cell in large isolated micro-well without complicated accessory equipment.Biomicrofluidics · 2018Article
- Review
- A Microfluidic Platform for High-throughput Single-cell Isolation and Culture.Journal of visualized experiments : JoVE · 2016Article
- Digital Microfluidics for Manipulation and Analysis of a Single Cell.International journal of molecular sciences · 2015Review
- Short-term expansion of breast circulating cancer cells predicts response to anti-cancer therapy.Oncotarget · 2015Article
- Nanowell-based immunoassays for measuring single-cell secretion: characterization of transport and surface binding.Analytical chemistry · 2014Article
- Bacterial nanoscale cultures for phenotypic multiplexed antibiotic susceptibility testing.Journal of clinical microbiology · 2014Article
- Advances in high-throughput single-cell microtechnologies.Current opinion in biotechnology · 2014Review
- On-chip biomedical imaging.IEEE reviews in biomedical engineering · 2013Review
- A hybrid microfluidic platform for cell-based assays via diffusive and convective trans-membrane perfusion.Biomicrofluidics · 2013Article
- Microfluidic device for generating a stepwise concentration gradient on a microwell slide for cell analysis.Biomicrofluidics · 2013Article
- Article
- Spontaneous differentiation of porcine neural progenitors in vitro.Cytotechnology · 2011Article
- Rise of the micromachines: microfluidics and the future of cytometry.Methods in cell biology · 2011Article
- High throughput screening to investigate the interaction of stem cells with their extracellular microenvironment.OrganogenesisReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
With recent findings on the role of reprogramming factors on stem cells, in vitro screening assays for studying (de)-differentiation is of great interest. We developed a miniaturized stem cell screening chip that is easily accessible and provides means of rapidly studying thousands of individual stem/progenitor cell samples, using low reagent volumes. For example, screening of 700,000 substances would take less than two days, using this platform combined with a conventional bio-imaging system. The microwell chip has standard slide format and consists of 672 wells in total. Each well holds 500 nl, a volume small enough to drastically decrease reagent costs but large enough to allow utilization of standard laboratory equipment. Results presented here include weeklong culturing and differentiation assays of mouse embryonic stem cells, mouse adult neural stem cells, and human embryonic stem cells. The possibility to either maintain the cells as stem/progenitor cells or to study cell differentiation of stem/progenitor cells over time is demonstrated. Clonality is critical for stem cell research, and was accomplished in the microwell chips by isolation and clonal analysis of single mouse embryonic stem cells using flow cytometric cell-sorting. Protocols for practical handling of the microwell chips are presented, describing a rapid and user-friendly method for the simultaneous study of thousands of stem cell cultures in small microwells. This microwell chip has high potential for a wide range of applications, for example directed differentiation assays and screening of reprogramming factors, opening up considerable opportunities in the stem cell field.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.