ArticleApplied and environmental microbiology2009
Edwardsiella ictaluri encodes an acid-activated urease that is required for intracellular replication in channel catfish (Ictalurus punctatus) macrophages.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
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- The RNA chaperone Hfq has a multifaceted role inFrontiers in cellular and infection microbiology · 2024Article
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- Diverse Bacteriophages Infecting the Bacterial Striped Catfish PathogenMicroorganisms · 2021Article
- Hemolysin Co-regulated Family Proteins Hcp1 and Hcp2 Contribute toFrontiers in veterinary science · 2021Article
- Early Intracellular Trafficking and Subsequent Activity of Programmed Cell Death in Channel Catfish Macrophages Infected withMicroorganisms · 2020Article
- Comparative genomics of the fish pathogensMicrobial genomics · 2020Article
- Gene expression of putative type VI secretion system (T6SS) genes in the emergent fish pathogen Francisella noatunensis subsp. orientalis in different physiochemical conditions.BMC microbiology · 2019Article
- First report of the occurrence and whole-genome characterization of Edwardsiella tarda in the false killer whale (Pseudorca crassidens).The Journal of veterinary medical science · 2018Article
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- Regulation of Type III Secretion of Translocon and Effector Proteins by the EsaB/EsaL/EsaM Complex in Edwardsiella tarda.Infection and immunity · 2017Article
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- The Sit-and-Wait Hypothesis in Bacterial Pathogens: A Theoretical Study of Durability and Virulence.Frontiers in microbiology · 2017Article
- Modulation of vacuolar pH is required for replication of Edwardsiella ictaluri in channel catfish macrophages.Infection and immunity · 2014Article
- Mechanisms of intrinsic resistance to antimicrobial peptides of Edwardsiella ictaluri and its influence on fish gut inflammation and virulence.Microbiology (Reading, England) · 2013Article
- Early interactions of Edwardsiella ictaluri, with Pangasianodon catfish and its invasive ability in cell lines.Veterinary research communications · 2012Article
- Regulation of the Edwardsiella ictaluri type III secretion system by pH and phosphate concentration through EsrA, EsrB, and EsrC.Applied and environmental microbiology · 2011Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Genomic analysis indicated that Edwardsiella ictaluri encodes a putative urease pathogenicity island containing the products of nine open reading frames, including urea and ammonium transporters. In vitro studies with wild-type E. ictaluri and a ureG::kan urease mutant strain indicated that E. ictaluri is significantly tolerant of acid conditions (pH 3.0) but that urease activity is not required for acid tolerance. Growth studies demonstrated that E. ictaluri is unable to grow at pH 5 in the absence of urea but is able to elevate the environmental pH from pH 5 to pH 7 and grow when exogenous urea is available. Substantial production of ammonia was observed for wild-type E. ictaluri in vitro in the presence of urea at low pH, and optimal activity occurred at pH 2 to 3. No ammonia production was detected for the urease mutant. Proteomic analysis with two-dimensional gel electrophoresis indicated that urease proteins are expressed at both pH 5 and pH 7, although urease activity is detectable only at pH 5. Urease was not required for initial invasion of catfish but was required for subsequent proliferation and virulence. Urease was not required for initial uptake or survival in head kidney-derived macrophages but was required for intracellular replication. Intracellular replication of wild-type E. ictaluri was significantly enhanced when urea was present, indicating that urease plays an important role in intracellular survival and replication, possibly through neutralization of the acidic environment of the phagosome.
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