ArticleJournal of bacteriology2009
Motility and flagellar glycosylation in Clostridium difficile.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
88 citing papers in PubMed, 146 citations in OpenAlex.
- Genomic and phenotypic insight intoEmerging microbes & infections · 2026Article
- A programmed reset mechanism primesScience advances · 2026Article
- Flagellar glycosylation with pseudaminic acids is widespread in the genus Clostridium.BMC microbiology · 2026Article
- Commensal bacterial glycosylation at the interface of host-bacteria interactions.Gut microbes · 2025Review
- Review
- Elucidation of the Glycan Structure of the b-type Flagellin ofACS infectious diseases · 2025Article
- Glycosylation of oral bacteria in modulating adhesion and biofilm formation.Journal of oral microbiology · 2025Review
- Allele-linked divergence in SlpA and TcdB drives distinct immune and cytotoxic responses that distinguish ST01 from non-ST01 strains in Clade 2 Clostridioides difficile.Access microbiology · 2025Article
- A novel regulator CdsR negatively regulates cell motility in Bacillus thuringiensis.Scientific reports · 2024Article
- Harnessing the acceptor substrate promiscuity of Clostridium botulinum Maf glycosyltransferase to glyco-engineer mini-flagellin protein chimeras.Communications biology · 2024Article
- Review
- Clostridioides difficile Biofilm.Advances in experimental medicine and biology · 2024Article
- Recent Developments and Application of Mass Spectrometry Imaging in N-Glycosylation Studies: An Overview.Mass spectrometry (Tokyo, Japan) · 2024Review
- Revised Model for the Type A Glycan Biosynthetic Pathway inACS infectious diseases · 2023Article
- Commensal bacteria signal through TLR5 and AhR to improve barrier integrity and prevent allergic responses to food.Cell reports · 2023Article
- Review
- Esculin hydrolysis negative and TcdA-only producing strains of Clostridium (Clostridioides) difficile from the environment in Western Australia.Journal of applied microbiology · 2022Article
- Heterogeneous glycosylation and methylation of the Aeromonas caviae flagellin.MicrobiologyOpen · 2022Article
- Destination and Specific Impact of Different Bile Acids in the Intestinal PathogenFrontiers in microbiology · 2022Article
- Biofilm regulation in Clostridioides difficile: Novel systems linked to hypervirulence.PLoS pathogens · 2021Review
28 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, intact flagellin proteins were purified from strains of Clostridium difficile and analyzed using quadrupole time of flight and linear ion trap mass spectrometers. Top-down studies showed the flagellin proteins to have a mass greater than that predicted from the corresponding gene sequence. These top-down studies revealed marker ions characteristic of glycan modifications. Additionally, diversity in the observed masses of glycan modifications was seen between strains. Electron transfer dissociation mass spectrometry was used to demonstrate that the glycan was attached to the flagellin protein backbone in O linkage via a HexNAc residue in all strains examined. Bioinformatic analysis of C. difficile genomes revealed diversity with respect to glycan biosynthesis gene content within the flagellar biosynthesis locus, likely reflected by the observed flagellar glycan diversity. In C. difficile strain 630, insertional inactivation of a glycosyltransferase gene (CD0240) present in all sequenced genomes resulted in an inability to produce flagellar filaments at the cell surface and only minor amounts of unmodified flagellin protein.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.