ArticlePlant physiology2009
The metabolic role of the legume endosperm: a noninvasive imaging study.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
- A survey of triploid block inconsistency: potential mechanisms and evolutionary implications on polyploid speciation.Journal of experimental botany · 2025Pooled it
- A holistic visualization for quality of Chinese materia medica: Structural and metabolic visualization by magnetic resonance imaging.Journal of pharmaceutical analysis · 2024Review
- Metabolic imaging in living plants: A promising field for chemical exchange saturation transfer (CEST) MRI.Science advances · 2024Article
- Carbon fluxes and environmental interactions during legume development, with a specific focus on Pisum sativum.Physiologia plantarum · 2022Review
- Temporal Control of Seed Development in Dicots: Molecular Bases, Ecological Impact and Possible Evolutionary Ramifications.International journal of molecular sciences · 2021Review
- Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis.Journal of experimental botany · 2020Article
- Physiological Profiling of Embryos and Dormant Seeds in TwoFrontiers in plant science · 2020Article
- Family plot: the impact of the endosperm and other extra-embryonic seed tissues on angiosperm zygotic embryogenesis.F1000Research · 2020Review
- Dye- and fluorescence-based assay to characterize symplastic and apoplastic trafficking in soybean (Glycime max L.) endosperm.Botanical studies · 2019Article
- The Role of Persulfide Metabolism During Arabidopsis Seed Development Under Light and Dark Conditions.Frontiers in plant science · 2018Article
- Identification ofFrontiers in plant science · 2018Article
- In Vivo Non-Destructive Monitoring of Capsicum Annuum Seed Growth with Diverse NaCl Concentrations Using Optical Detection Technique.Sensors (Basel, Switzerland) · 2017Article
- Macro optical projection tomography for large scale 3D imaging of plant structures and gene activity.Journal of experimental botany · 2017Article
- Identification ofFrontiers in plant science · 2017Article
- Ascorbate efflux as a new strategy for iron reduction and transport in plants.The Journal of biological chemistry · 2014Article
- Physical, metabolic and developmental functions of the seed coat.Frontiers in plant science · 2014Review
- Genomic dissection of the seed.Frontiers in plant science · 2014Review
- Effects of the magnetic resonance imaging contrast agent Gd-DTPA on plant growth and root imaging in rice.PloS one · 2014Article
- Seed architecture shapes embryo metabolism in oilseed rape.The Plant cell · 2013Article
- A noninvasive platform for imaging and quantifying oil storage in submillimeter tobacco seed.Plant physiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although essential for normal seed development in the legumes, the metabolic role of the endosperm remains uncertain. We designed noninvasive nuclear magnetic resonance tools for the in vivo study of key metabolites in the transient liquid endosperm of intact pea (Pisum sativum) seeds. The steady-state levels of sucrose, glutamine, and alanine could be monitored and their distribution within the embryo sac visualized. Seed structure was digitalized as a three-dimensional model, providing volume information for distinct seed organs. The nuclear magnetic resonance method, combined with laser microdissection, isotope labeling, in situ hybridization, and electron microscopy, was used to contrast the wild-type endosperm with that of a mutant in which embryo growth is retarded. Expression of sequences encoding amino acid and sucrose transporters was up-regulated earlier in the endosperm than in the embryo, and this activity led to the accumulation of soluble metabolites in the endosperm vacuole. The endosperm provides a temporary source of nutrition, permits space for embryo growth, and acts as a buffer between the maternal organism and its offspring. The concentration of sucrose in the endosperm vacuole is developmentally controlled, while the total amount accumulated depends on the growth of the embryo. The endosperm concentration of glutamine is a limiting factor for protein storage. The properties of the endosperm ensure that the young embryo develops within a homeostatic environment, necessary to sustain embryogenesis. We argue for a degree of metabolite-mediated control exerted by the endosperm on the growth of, and assimilate storage by, the embryo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.