ArticleDiagnostic microbiology and infectious disease2009
A duplex real-time reverse transcriptase polymerase chain reaction assay for the detection of California serogroup and Cache Valley viruses.
Article in Diagnostic microbiology and infectious disease, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- RNA metagenomic profiling of mosquito viromes associated with Vector-Borne diseases in Quebec, Canada.PloS one · 2026Article
- Zoonotic Abortifacient Agents in Bovine Abortion: Diagnostic Assessment of 125 Cases (2015-2017).Veterinary medicine and science · 2025Article
- A six-year epidemiological study of selected zoonotic abortifacient agents in ovine and caprine foetuses in Türkiye.Epidemiology and infection · 2024Article
- A cost-effective RNA extraction and RT-qPCR approach to detect California serogroup viruses from pooled mosquito samples.Scientific reports · 2024Article
- Development of Real-Time Molecular Assays for the Detection of Wesselsbron Virus in Africa.Microorganisms · 2022Article
- Monoclonal antibodies to Cache Valley virus for serological diagnosis.PLoS neglected tropical diseases · 2022Article
- Diagnostic Approach for Arboviral Infections in the United States.Journal of clinical microbiology · 2020Review
- Article
- Culex tarsalis is a competent vector species for Cache Valley virus.Parasites & vectors · 2018Article
- A novel cause of chronic viral meningoencephalitis: Cache Valley virus.Annals of neurology · 2017Article
- Cache valley virus in a patient diagnosed with aseptic meningitis.Journal of clinical microbiology · 2013Article
- Orthobunyavirus antibodies in humans, Yucatan Peninsula, Mexico.Emerging infectious diseases · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A duplex TaqMan real-time reverse transcriptase polymerase chain reaction (PCR) assay was developed for the detection of California (CAL) serogroup viruses and Cache Valley virus (CVV), for use in human surveillance. The targets selected for the assay were the sequences encoding the nucleocapsid protein of CAL and the G1 glycoprotein of CVV. Conserved regions were selected by aligning genetic sequences from various strains available in the GenBank database. Primers and probes were selected in conserved regions. The assay sensitivity was 75 gene copies (gc)/reaction for CAL serogroup viruses and 30 gc/reaction for CVV. The performance of the assay was linear over at least 6 log(10) gc. The assay was specific, given that it did not cross-react with a variety of pathogens. It did, however, detect 11 viruses within the CAL serogroup and 12 CVV isolates. The use of an internal control ensured that possible inefficiency in nucleic acid extraction or PCR inhibition would be detected.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.