ArticleStructure (London, England : 1993)2009
Structural polymorphism of the ParM filament and dynamic instability.
Article in Structure (London, England : 1993), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Catching a Walker in the Act-DNA Partitioning by ParA Family of Proteins.Frontiers in microbiology · 2022Review
- Bioinformatics Analysis of Actin Molecules: Why Quantity Does Not Translate Into Quality?Frontiers in genetics · 2020Article
- Conservation of conformational dynamics across prokaryotic actins.PLoS computational biology · 2019Article
- Cryo-EM structure of the bacterial actin AlfA reveals unique assembly and ATP-binding interactions and the absence of a conserved subdomain.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Polymerization Dynamics of the Prophage-Encoded Actin-Like Protein AlpC Is Influenced by the DNA-Binding Adapter AlpA.Frontiers in microbiology · 2017Article
- Archaeal actin from a hyperthermophile forms a single-stranded filament.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Bacterial actins and their diversity.Biochemistry · 2013Review
- Structure of the ParM filament at 8.5Å resolution.Journal of structural biology · 2013Article
- The bacterial actin MamK: in vitro assembly behavior and filament architecture.The Journal of biological chemistry · 2013Article
- The bacterial cytoskeleton: more than twisted filaments.Current opinion in cell biology · 2013Review
- Structure and function of the adhesive type IV pilus of Sulfolobus acidocaldarius.Environmental microbiology · 2012Article
- Are ParM filaments polar or bipolar?Journal of molecular biology · 2012Article
- Bacterial actin homolog ParM: arguments for an apolar, antiparallel double helix.Journal of molecular biology · 2012Article
- Filaments from Ignicoccus hospitalis show diversity of packing in proteins containing N-terminal type IV pilin helices.Journal of molecular biology · 2012Article
- Novel actin-like filament structure from Clostridium tetani.The Journal of biological chemistry · 2012Article
- Structure of the Vibrio cholerae Type IVb Pilus and stability comparison with the Neisseria gonorrhoeae type IVa pilus.Journal of molecular biology · 2012Article
- Actin filaments as tension sensors.Current biology : CB · 2012Review
- Architecture and assembly of a divergent member of the ParM family of bacterial actin-like proteins.The Journal of biological chemistry · 2011Article
- Actin structure and function.Annual review of biophysics · 2011Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Segregation of the R1 plasmid in bacteria relies on ParM, an actin homolog that segregates plasmids by switching between cycles of polymerization and depolymerization. We find similar polymerization kinetics and stability in the presence of either ATP or GTP and a 10-fold affinity preference for ATP over GTP. We used electron cryo-microscopy to evaluate the heterogeneity within ParM filaments. In addition to variable twist, ParM has variable axial rise, and both parameters are coupled. Subunits in the same ParM filaments can exist in two different structural states, with the nucleotide-binding cleft closed or open, and the bound nucleotide biases the distribution of states. The interface between protomers is different between these states, and in neither state is it similar to F-actin. Our results suggest that the closed state of the cleft is required but not sufficient for ParM polymerization, and provide a structural basis for the dynamic instability of ParM filaments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.