ArticleClinical biochemistry2010
Measurement of glucose and fructose in clinical samples using gas chromatography/mass spectrometry.
Article in Clinical biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 110 citations in OpenAlex.
- Consumption of Fructose by Cultured Primary Rat Astrocytes.Neurochemical research · 2026Article
- Metabolic and regulatory adaptations of Staphylococcus epidermidis to host stresses.FEMS microbiology reviews · 2026Review
- Studies on glucose detection using graphene quantum dots prepared by hydrothermal method.Scientific reports · 2025Article
- Fructose metabolism and its roles in metabolic diseases, inflammatory diseases, and cancer.Molecular biomedicine · 2025Review
- Article
- Regulation of Fructose Metabolism in Nonalcoholic Fatty Liver Disease.Biomolecules · 2024Review
- Fructose Reduces Mitochondrial Metabolism and Increases Extracellular BCAA during Insulin Resistance in C2C12 Myotubes.Nutrients · 2024Article
- Green synthesized gold nanoparticles and CuO-based nonenzymatic sensor for saliva glucose monitoring.RSC advances · 2024Article
- Glucose Oxidase Loading in Ordered Porous Aluminosilicates: Exploring the Potential of Surface Modification for Electrochemical Glucose Sensing.Chemistry of materials : a publication of the American Chemical Society · 2023Article
- Sugar Molecules Detection via CNanomaterials (Basel, Switzerland) · 2023Article
- Synthesis and Characterization of TiOBiosensors · 2022Article
- Immunomodulatory Properties of Polysaccharide-Rich Young Green Barley (Molecules (Basel, Switzerland) · 2022Article
- Fructose metabolism and its role in pig production: A mini-review.Frontiers in nutrition · 2022Review
- Enzyme-Free Electrochemical Sensors forFrontiers in plant science · 2022Article
- Fructose and metabolic diseases: too much to be good.Chinese medical journal · 2021Article
- DNA methylation during human adipogenesis and the impact of fructose.Genes & nutrition · 2020Article
- Fructose contributes to the Warburg effect for cancer growth.Cancer & metabolism · 2020Review
- Review
- 3-Hydroxyphenylboronic Acid-Based Carbon Dot Sensors for Fructose Sensing.Journal of fluorescence · 2019Article
- Acute metabolic responses to high fructose corn syrup ingestion in adolescents with overweight/obesity and diabetes.Journal of nutrition & intermediary metabolism · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveThe impact of increased fructose consumption on carbohydrate metabolism is a topic of current interest, but determination of serum level has been hindered due to low concentration and interference from serum glucose. We are reporting a method for the quantification of glucose and fructose in clinical samples using gas chromatography/mass spectrometry (GC/MS). The accuracy and precision of GC/MS and an enzymatic assay were compared. DESIGN AND
methodsMass spectrometry fragmentation patterns of methyloxime peracetate derivatized aldose and ketose were determined. Unique fragments for glucose and fructose were used for quantitative analysis using isotope labeled recovery standards.
resultsMethyloxime peracetate derivatives of glucose and fructose showed characteristic loss of acetate (M-60) or ketene (M-42) under chemical ionization (CI). Under electron impact (EI) ionization, a unique C1-C2 fragment of glucose was formed, while a C1-C3 fragment was formed from keto-hexoses. These unique fragments were used in the quantitative assay of glucose and fructose in clinical samples. In clinical samples, the GC/MS assay has a lower limit of detection than that of the enzymatic assay. In plasma samples from patients evaluated for diabetes the average serum glucose and fructose were 6.19+/-2.72 mM and 46+/- 25.22 microM. Fructose concentrations in many of these samples were below the limit of detection of the enzymatic method.
conclusionDerivatization of aldose and ketose monosaccharides to their respective O-methyloxime acetates for GC/MS analysis is a facile method for determination of serum/plasma glucose and fructose samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.