ArticleBMC plant biology2009
Gene-based microsatellites for cassava (Manihot esculenta Crantz): prevalence, polymorphisms, and cross-taxa utility.
Article in BMC plant biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Development and Validation of Gene-Based SSR Markers in the GenusScientifica · 2023Article
- Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses.Nature communications · 2022Article
- Article
- Report on the development of putative functional SSR and SNP markers in passion fruits.BMC research notes · 2017Article
- Article
- Utilization of in silico EST-SSR markers for diversity studies in castor (Physiology and molecular biology of plants : an international journal of functional plant biology · 2016Article
- Transcriptome-enabled marker discovery and mapping of plastochron-related genes in Petunia spp.BMC genomics · 2015Article
- A genetic map of cassava (Manihot esculenta Crantz) with integrated physical mapping of immunity-related genes.BMC genomics · 2015Article
- Potential of SNP markers for the characterization of Brazilian cassava germplasm.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
- In silico genome comparison and distribution analysis of simple sequences repeats in cassava.International journal of genomics · 2014Article
- Article
- Comparison of leaf proteomes of cassava (Manihot esculenta Crantz) cultivar NZ199 diploid and autotetraploid genotypes.PloS one · 2014Article
- Transferability of molecular markers from major legumes to Lathyrus spp. for their application in mapping and diversity studies.Molecular biology reports · 2014Article
- Development of simple sequence repeat markers and diversity analysis in alfalfa (Medicago sativa L.).Molecular biology reports · 2013Article
- Genome-wide discovery and information resource development of DNA polymorphisms in cassava.PloS one · 2013Article
- Comparative morphology, biology and histology of reproductive development in three lines of Manihot esculenta Crantz (Euphorbiaceae: Crotonoideae).AoB PLANTS · 2013Article
- Phenotypic approaches to drought in cassava: review.Frontiers in physiology · 2013Article
- Identification, validation and high-throughput genotyping of transcribed gene SNPs in cassava.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- The Cassava Genome: Current Progress, Future Directions.Tropical plant biology · 2012Article
- Transcriptome sequencing of Hevea brasiliensis for development of microsatellite markers and construction of a genetic linkage map.DNA research : an international journal for rapid publication of reports on genes and genomes · 2011Article
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6 authors.
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Abstract
backgroundCassava (Manihot esculenta Crantz), a starchy root crop grown in tropical and subtropical climates, is the sixth most important crop in the world after wheat, rice, maize, potato and barley. The repertoire of simple sequence repeat (SSR) markers for cassava is limited and warrants a need for a larger number of polymorphic SSRs for germplasm characterization and breeding applications.
resultsA total of 846 putative microsatellites were identified in silico from an 8,577 cassava unigene set with an average density of one SSR every 7 kb. One hundred and ninety-two candidate SSRs were screened for polymorphism among a panel of cassava cultivars from Africa, Latin America and Asia, four wild Manihot species as well as two other important taxa in the Euphorbiaceae, leafy spurge (Euphorbia esula) and castor bean (Ricinus communis). Of 168 markers with clean amplification products, 124 (73.8%) displayed polymorphism based on high resolution agarose gels. Of 85 EST-SSR markers screened, 80 (94.1%) amplified alleles from one or more wild species (M epruinosa, M glaziovii, M brachyandra, M tripartita) whereas 13 (15.3%) amplified alleles from castor bean and 9 (10.6%) amplified alleles from leafy spurge; hence nearly all markers were transferable to wild relatives of M esculenta while only a fraction was transferable to the more distantly related taxa. In a subset of 20 EST-SSRs assessed by fluorescence-based genotyping the number of alleles per locus ranged from 2 to 10 with an average of 4.55 per locus. These markers had a polymorphism information content (PIC) from 0.19 to 0.75 with an average value of 0.55 and showed genetic relationships consistent with existing information on these genotypes.
conclusionA set of 124 new, unique polymorphic EST-SSRs was developed and characterized which extends the repertoire of SSR markers for cultivated cassava and its wild relatives. The markers show high PIC values and therefore will be useful for cultivar identification, taxonomic studies, and genetic mapping. The study further shows that mining ESTs is a highly efficient strategy for polymorphism detection within the cultivated cassava gene pool.
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