ArticleJournal of cellular physiology2010
Activation of nuclear factor kappa B in mammary epithelium promotes milk loss during mammary development and infection.
Article in Journal of cellular physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 70 citations in OpenAlex.
- Prediction of potential molecular markers of bovine mastitis by meta-analysis of differentially expressed genes using combined p value and robust rank aggregation.Tropical animal health and production · 2022Pooled it
- Across deserts and Andes: a genetic exploration of GlyCam-1 in domestic camelids.BMC veterinary research · 2025Article
- Regulation of Milk Production by the MAPK/ERK Pathway in Water Buffalo (Bubalus bubalis): Genomic and Molecular Insights.Veterinary medicine and science · 2025Article
- Th1 Cytokines Inhibit Acinar Morphogenesis and Milk Protein Expression in 3D Mammary Cultures.Biomedicines · 2025Article
- Pain in Dairy Cattle: A Narrative Review of the Need for Pain Control, Industry Practices and Stakeholder Expectations, and Opportunities.Animals : an open access journal from MDPI · 2025Review
- Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide.Animal biotechnology · 2024Review
- The effect of parity, breastfeeding history, and duration on clinical and pathological characteristics of breast cancer patients.Turkish journal of medical sciences · 2024Article
- Culture Models to Investigate Mechanisms of Milk Production and Blood-Milk Barrier in Mammary Epithelial Cells: a Review and a Protocol.Journal of mammary gland biology and neoplasia · 2023Review
- Calpains, the proteases of two faces controlling the epithelial homeostasis in mammary gland.Frontiers in cell and developmental biology · 2023Review
- A Novel TLR4-SYK Interaction Axis Plays an Essential Role in the Innate Immunity Response in Bovine Mammary Epithelial Cells.Biomedicines · 2022Article
- Breast cancers co-opt normal mechanisms of tolerance to promote immune evasion and metastasis.American journal of physiology. Cell physiology · 2022Review
- Lactose on the basolateral side of mammary epithelial cells inhibits milk production concomitantly with signal transducer and activator of transcription 5 inactivation.Cell and tissue research · 2022Article
- Immune defenses of the mammary gland epithelium of dairy ruminants.Frontiers in immunology · 2022Review
- Overview of Research Development on the Role of NF-κB Signaling in Mastitis.Animals : an open access journal from MDPI · 2020Review
- Postpartum Involution and Cancer: An Opportunity for Targeted Breast Cancer Prevention and Treatments?Cancer research · 2020Review
- PGC-1α induced browning promotes involution and inhibits lactation in mammary glands.Cellular and molecular life sciences : CMLS · 2019Article
- Buffalo milk transcriptome: A comparative analysis of early, mid and late lactation.Scientific reports · 2019Article
- Article
- Nucling, a novel apoptosis-associated protein, controls mammary gland involution by regulating NF-κB and STAT3.The Journal of biological chemistry · 2015Article
- Aberrant activation of NF-κB signaling in mammary epithelium leads to abnormal growth and ductal carcinoma in situ.BMC cancer · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
We investigated whether nuclear factor kappa B (NF-kappaB), which exhibits a regulated pattern of activity during murine mammary gland development, plays an important role during lactation and involution, when milk production ceases and the gland undergoes apoptosis and re-modeling. We generated a doxycycline inducible transgenic mouse model to activate NF-kappaB specifically in the mammary epithelium through expression of a constitutively active form of IKK2, the upstream kinase in the classical NF-kappaB signaling cascade. We found that activation of NF-kappaB during involution resulted in a more rapid reduction in milk levels and increased cleavage of caspase-3, an indicator of apoptosis. We also found that activation of NF-kappaB during lactation with no additional involution signals had a similar effect. The observation that NF-kappaB is a key regulator of milk production led us to investigate the role of NF-kappaB during mastitis, an infection of the mammary gland in which milk loss is observed. Mammary gland injection of E. coli LPS resulted in activation of NF-kappaB and milk loss during lactation. This milk loss was decreased by selective inhibition of NF-kappaB in mammary epithelium. Together, our data reveal that activation of NF-kappaB leads to milk clearance in the lactating mammary gland. Therefore, targeting of NF-kappaB signaling may prove therapeutic during mastitis in humans and could be beneficial for the dairy industry, where such infections have a major economic impact.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.