ArticleBMC genomics2009
Improvement of the clinical applicability of the Genomic Grade Index through a qRT-PCR test performed on frozen and formalin-fixed paraffin-embedded tissues.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 87 citations in OpenAlex.
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- Update Breast Cancer 2022 Part 3 - Early-Stage Breast Cancer.Geburtshilfe und Frauenheilkunde · 2022Article
- Tumor metabolism assessed by FDG-PET/CT and tumor proliferation assessed by genomic grade index to predict response to neoadjuvant chemotherapy in triple negative breast cancer.European journal of nuclear medicine and molecular imaging · 2018Article
- Association of microRNA-7 and its binding partner CDR1-AS with the prognosis and prediction of 1Scientific reports · 2018Article
- Genes and functions from breast cancer signatures.BMC cancer · 2018Article
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- Dissecting the Biological Heterogeneity within Hormone Receptor Positive HER2 Negative Breast Cancer by Gene Expression Markers Identifies Indolent Tumors within Late Stage Disease.Translational oncology · 2017Article
- Molecular stratification of early breast cancer identifies drug targets to drive stratified medicine.NPJ breast cancer · 2017Article
- Tumour sampling method can significantly influence gene expression profiles derived from neoadjuvant window studies.Scientific reports · 2016Article
- Mitochondrial DNA content in breast cancer: Impact on in vitro and in vivo phenotype and patient prognosis.Oncotarget · 2016Article
- Gene profiling and circulating tumor cells as biomarker to prognostic of patients with locoregional breast cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Multigene prognostic tests in breast cancer: past, present, future.Breast cancer research : BCR · 2015Review
- Breast cancer intrinsic subtype classification, clinical use and future trends.American journal of cancer research · 2015Review
- Molecular tests as prognostic factors in breast cancer.Virchows Archiv : an international journal of pathology · 2014Review
- Multigene Assays for Classification, Prognosis, and Prediction in Breast Cancer: a Critical Review on the Background and Clinical Utility.Geburtshilfe und Frauenheilkunde · 2013Article
- KLF6-SV1 drives breast cancer metastasis and is associated with poor survival.Science translational medicine · 2013Article
- Gene expression profiling in breast cancer.American journal of translational research · 2013Article
- The CIN4 chromosomal instability qPCR classifier defines tumor aneuploidy and stratifies outcome in grade 2 breast cancer.PloS one · 2013Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProliferation and tumor differentiation captured by the genomic grade index (GGI) are important prognostic indicators in breast cancer (BC) especially for the estrogen receptor positive (ER+) disease. The aims of this study were to convert this microarray index to a qRT-PCR assay (PCR-GGI), which could be realized on formalin fixed paraffin embedded samples (FFPE), and to assess its prognostic performance and predictive value of clinical benefit in early and advanced ER+ BC patients treated with tamoxifen.
methodsThe accuracy and concordance of the PCR-GGI with the GGI was assessed using BC patients for which frozen and FFPE tissues as well as microarray data were available (n = 19). The evaluation of the prognostic value of the PCR-GGI was assessed on FFPE material using a consecutive series of 212 systemically treated early BC patients. The predictive performance for tamoxifen benefit was assessed using two ER+ BC populations treated either with adjuvant tamoxifen only (n = 77+139) or first-line tamoxifen for advanced disease (n = 270).
resultsThe PCR-GGI is based on the expression of 8 genes (4 representative of the GGI and 4 reference genes). A significant correlation was observed between the microarray-derived GGI and the qRT-PCR assay using frozen (rho = 0.95, p < 10E-06) and FFPE material (rho = 0.89, p < 10E-06). The prognostic performance of the PCR-GGI was confirmed on FFPE samples (HRunivar. = 1.89; [95CI:1.01-3.54], p = 0.05). The PCR-GGI further identified two subgroups of patients with statistically different time to distant metastasis free survival (DMFS) across the two cohorts of ER+ BC patients treated with adjuvant tamoxifen. Additionally, the PCR-GGI was associated with response to tamoxifen in the advanced setting (HRunivar. = 1.98; [95CI:1.51-2.59], p = 6.9E-07).
conclusionPCR-GGI recapitulates in an accurate and reproducible manner the performances of the GGI using frozen and FFPE samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.