ArticlePloS one2009
Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- A brain stem circuit integrating reflexive and anticipatory salivation.Cell reports · 2026Article
- Transsynaptic viral tools in neural circuit analysis: from anatomical mapping to functional interrogation.Frontiers in neural circuits · 2026Review
- Advances in molecular epidemiology and detection methods of pseudorabies virus.Discover nano · 2025Review
- Transneuronal Circuit Analysis with Pseudorabies Viruses.Current protocols · 2023Review
- Imaging in vivo acetylcholine release in the peripheral nervous system with a fluorescent nanosensor.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Connect-seq to superimpose molecular on anatomical neural circuit maps.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A corticopontine circuit for initiation of urination.Nature neuroscience · 2018Article
- Changes in membrane properties of rat deep cerebellar nuclear projection neurons during acquisition of eyeblink conditioning.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Review
- Evaluation of the impact of ul54 gene-deletion on the global transcription and DNA replication of pseudorabies virus.Archives of virology · 2017Article
- Characterization of the Dynamic Transcriptome of a Herpesvirus with Long-read Single Molecule Real-Time Sequencing.Scientific reports · 2017Article
- Implications for bidirectional signaling between afferent nerves and urothelial cells-ICI-RS 2014.Neurourology and urodynamics · 2016Review
- In vivo imaging of alphaherpesvirus infection reveals synchronized activity dependent on axonal sorting of viral proteins.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Revealing the secrets of neuronal circuits with recombinant rabies virus technology.Frontiers in neural circuits · 2013Review
- Alphaherpesvirus infection disrupts mitochondrial transport in neurons.Cell host & microbe · 2012Article
- A wide extent of inter-strain diversity in virulent and vaccine strains of alphaherpesviruses.PLoS pathogens · 2011Article
- Preparation of viral DNA from nucleocapsids.Journal of visualized experiments : JoVE · 2011Article
- Article
- Microdissection of neural networks by conditional reporter expression from a Brainbow herpesvirus.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The effects of viral load on pseudorabies virus gene expression.BMC microbiology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The study of coordinated activity in neuronal circuits has been challenging without a method to simultaneously report activity and connectivity. Here we present the first use of pseudorabies virus (PRV), which spreads through synaptically connected neurons, to express a fluorescent calcium indicator protein and monitor neuronal activity in a living animal. Fluorescence signals were proportional to action potential number and could reliably detect single action potentials in vitro. With two-photon imaging in vivo, we observed both spontaneous and stimulated activity in neurons of infected murine peripheral autonomic submandibular ganglia (SMG). We optically recorded the SMG response in the salivary circuit to direct electrical stimulation of the presynaptic axons and to physiologically relevant sensory stimulation of the oral cavity. During a time window of 48 hours after inoculation, few spontaneous transients occurred. By 72 hours, we identified more frequent and prolonged spontaneous calcium transients, suggestive of neuronal or tissue responses to infection that influence calcium signaling. Our work establishes in vivo investigation of physiological neuronal circuit activity and subsequent effects of infection with single cell resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.