ArticleMolecular & cellular proteomics : MCP2009
In vitro and in vivo protein-bound tyrosine nitration characterized by diagonal chromatography.
Article in Molecular & cellular proteomics : MCP, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 92 citations in OpenAlex.
- Nitration in cancer signaling.Redox biology · 2026Review
- Creating a Selective Nanobody Against 3-Nitrotyrosine Containing Proteins.Frontiers in chemistry · 2022Article
- Unraveling the effects of peroxiredoxin 2 nitration; role of C-terminal tyrosine 193.Free radical biology & medicine · 2019Article
- Genetically Encoded Protein Tyrosine Nitration in Mammalian Cells.ACS chemical biology · 2019Article
- Switchable Nitroproteome States ofFrontiers in microbiology · 2019Article
- Selective Affinity Enrichment of Nitrotyrosine-Containing Peptides for Quantitative Analysis in Complex Samples.Journal of proteome research · 2017Article
- Structural changes upon peroxynitrite-mediated nitration of peroxiredoxin 2; nitrated Prx2 resembles its disulfide-oxidized form.Archives of biochemistry and biophysics · 2016Article
- Mass spectrometry-based methods for identifying oxidized proteins in disease: advances and challenges.Biomolecules · 2015Review
- ANSID: A Solid-Phase Proteomic Approach for Identification and Relative Quantification of Aromatic Nitration Sites.Frontiers in chemistry · 2015Article
- Systematic analysis of the in situ crosstalk of tyrosine modifications reveals no additional natural selection on multiply modified residues.Scientific reports · 2014Article
- Proteome Changes Induced by Imatinib and Novel Imatinib Derivatives in K562 Human Chronic Myeloid Leukemia Cells.Proteomes · 2014Article
- Nitration transforms a sensitive peroxiredoxin 2 into a more active and robust peroxidase.The Journal of biological chemistry · 2014Article
- Selective chemoprecipitation to enrich nitropeptides from complex proteomes for mass-spectrometric analysis.Nature protocols · 2014Article
- iNitro-Tyr: prediction of nitrotyrosine sites in proteins with general pseudo amino acid composition.PloS one · 2014Article
- Proteomic approaches to analyze protein tyrosine nitration.Antioxidants & redox signaling · 2013Review
- Mapping proteolytic processing in the secretome of gastric cancer-associated myofibroblasts reveals activation of MMP-1, MMP-2, and MMP-3.Journal of proteome research · 2013Article
- Activation of ADF/cofilin by phosphorylation-regulated Slingshot phosphatase is required for the meiotic spindle assembly in Xenopus laevis oocytes.Molecular biology of the cell · 2013Article
- Bioinformatics analysis reveals biophysical and evolutionary insights into the 3-nitrotyrosine post-translational modification in the human proteome.Open biology · 2013Article
- Computational study of the effects of protein tyrosine nitrations on the catalytic activity of human thymidylate synthase.Journal of computer-aided molecular design · 2013Article
- Conversion of 3-nitrotyrosine to 3-aminotyrosine residues facilitates mapping of tyrosine nitration in proteins by electrospray ionization-tandem mass spectrometry using electron capture dissociation.Journal of mass spectrometry : JMS · 2012Article
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new proteomics technique for analyzing 3-nitrotyrosine-containing peptides is presented here. This technique is based on the combined fractional diagonal chromatography peptide isolation procedures by which specific classes of peptides are isolated following a series of identical reverse-phase HPLC separation steps. Here dithionite is used to reduce 3-nitrotyrosine to 3-aminotyrosine peptides, which thereby become more hydrophilic. Our combined fractional diagonal chromatography technique was first applied to characterize tyrosine nitration in tetranitromethane-modified BSA and further led to a high quality list of 335 tyrosine nitration sites in 267 proteins in a peroxynitrite-treated lysate of human Jurkat cells. We then analyzed a serum sample of a C57BL6/J mouse in which septic shock was induced by intravenous Salmonella infection and identified six in vivo nitration events in four serum proteins, thereby illustrating that our technique is sufficiently sensitive to identify rare in vivo tyrosine nitration sites in a very complex background.
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