ArticleMolecular biology of the cell2009
Unbalancing the phosphatidylinositol-4,5-bisphosphate-cofilin interaction impairs cell steering.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Review of PIP2 in Cellular Signaling, Functions and Diseases.International journal of molecular sciences · 2020Review
- Cofilin, a Master Node Regulating Cytoskeletal Pathogenesis in Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2019Review
- The PET and LIM1-2 domains of testin contribute to intramolecular and homodimeric interactions.PloS one · 2017Article
- Chronophin coordinates cell leading edge dynamics by controlling active cofilin levels.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cofilin: a redox sensitive mediator of actin dynamics during T-cell activation and migration.Immunological reviews · 2013Review
- Functions of cofilin in cell locomotion and invasion.Nature reviews. Molecular cell biology · 2013Review
- Phosphatidylinositol 4,5-bisphosphate: targeted production and signaling.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Review
- Alphaherpesviral US3 kinase induces cofilin dephosphorylation to reorganize the actin cytoskeleton.Journal of virology · 2013Article
- Considering protonation as a posttranslational modification regulating protein structure and function.Annual review of biophysics · 2013Review
- Activity of PLCε contributes to chemotaxis of fibroblasts towards PDGF.Journal of cell science · 2012Article
- Cells lacking β-actin are genetically reprogrammed and maintain conditional migratory capacity.Molecular & cellular proteomics : MCP · 2012Article
- cAMP regulates DEP domain-mediated binding of the guanine nucleotide exchange factor Epac1 to phosphatidic acid at the plasma membrane.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cortactin phosphorylation regulates cell invasion through a pH-dependent pathway.The Journal of cell biology · 2011Article
- ADF/cofilin binds phosphoinositides in a multivalent manner to act as a PIP(2)-density sensor.Biophysical journal · 2010Article
- Phosphoinositide signalling in cancer: beyond PI3K and PTEN.Nature reviews. Cancer · 2010Review
- ADF/cofilin: a functional node in cell biology.Trends in cell biology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
Abstract
Cofilin is a key player in actin dynamics during cell migration. Its activity is regulated by (de)phosphorylation, pH, and binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P(2)]. Here, we here use a human cofilin-1 (D122K) mutant with increased binding affinity for PI(4,5)P(2) and slower release from the plasma membrane to study the role of the PI(4,5)P(2)-cofilin interaction in migrating cells. In fibroblasts in a background of endogenous cofilin, D122K cofilin expression negatively affects cell turning frequency. In carcinoma cells with down-regulated endogenous cofilin, D122K cofilin neither rescues the drastic morphological defects nor restores the effects in cell turning capacity, unlike what has been reported for wild-type cofilin. In cofilin knockdown cells, D122K cofilin expression promotes outgrowth of an existing lamellipod in response to epidermal growth factor (EGF) but does not result in initiation of new lamellipodia. This indicates that, next to phospho- and pH regulation, the normal release kinetics of cofilin from PI(4,5)P(2) is crucial as a local activation switch for lamellipodia initiation and as a signal for migrating cells to change direction in response to external stimuli. Our results demonstrate that the PI(4,5)P(2) regulatory mechanism, that is governed by EGF-dependent phospholipase C activation, is a determinant for the spatial and temporal control of cofilin activation required for lamellipodia initiation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.