ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2009
Functional significance of muscarinic receptor expression within the proximal and distal rat vagina.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- The impact of pharmacotherapy on sexual function in female patients being treated for idiopathic overactive bladder: a systematic review.BMC women's health · 2024Pooled it
- Advances in vaginal bioengineering: Applications, techniques, and needs.Current research in physiology · 2023Review
- Contractile Properties of Vaginal Tissue.Journal of biomechanical engineering · 2020Review
- Smooth muscle regional contribution to vaginal wall function.Interface focus · 2019Article
- Nerve-induced responses of mouse vaginal smooth muscle.Pflugers Archiv : European journal of physiology · 2017Article
- Phasic contractions of the mouse vagina and cervix at different phases of the estrus cycle and during late pregnancy.PloS one · 2014Article
- Effect of estrogen on molecular and functional characteristics of the rodent vaginal muscularis.The journal of sexual medicine · 2013Article
- Regional differences in rat vaginal smooth muscle contractility and morphology.Reproductive sciences (Thousand Oaks, Calif.) · 2013Article
- Adaptive plasticity of vaginal innervation in term pregnant rats.Reproductive sciences (Thousand Oaks, Calif.) · 2011Article
- Contractile response of human anterior vaginal muscularis in women with and without pelvic organ prolapse.Reproductive sciences (Thousand Oaks, Calif.) · 2011Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Information regarding the role of cholinergic nerves in mediating vaginal smooth muscle contraction is sparse, and in vitro studies of the effects of muscarinic agonists on vaginal smooth muscle are discrepant. The goal of this study was to determine the expression of muscarinic receptors in the vaginal wall of the rat. In addition, we sought to determine the effect of the muscarinic receptor agonist carbachol on contractility and inositol phosphate production of the proximal and distal rat vaginal muscularis. RT-PCR analysis indicated that both M(2) and M(3) receptor transcripts were expressed within the proximal and distal rat vagina. Carbachol dose-dependently (10(-7)-10(-4) M) contracted the rat vaginal muscularis with a greater maximal contractile response in the proximal vagina (P < 0.01) compared with the distal vagina. The contractile responses of the rat vaginal muscularis to carbachol were dose dependently inhibited by the M(3) antagonist para-fluoro-hexahydrosiladefenidol, and a pK(B) of 7.78 and 7.95 was calculated for the proximal and distal vagina, respectively. Inositol phosphate production was significantly increased in both regions of the vagina following 20-min exposure to 50 muM carbachol with higher levels detected in the proximal vagina compared with the distal (P < 0.05). Preliminary experiments indicated the presence of M(2) and M(3) receptors in the human vaginal muscularis as well as contraction of human vaginal muscularis to carbachol, indicating that our animal studies are relevant to human tissue. Our results provide strong evidence for the functional significance of M(3) receptor expression in the vaginal muscularis.
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