ArticleJournal of virology2009
Detection of PrPsc in blood from sheep infected with the scrapie and bovine spongiform encephalopathy agents.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- A comparison of four technologies for detecting p53 aggregates in ovarian cancer.Frontiers in oncology · 2022Article
- Detection of Pathognomonic Biomarker PrPBiomolecules · 2020Review
- Prenatal transmission of scrapie in sheep and goats: A case study for veterinary public health.Open veterinary journal · 2016Review
- Classical natural ovine scrapie prions detected in practical volumes of blood by lamb and transgenic mouse bioassays.Journal of veterinary science · 2015Article
- First demonstration of transmissible spongiform encephalopathy-associated prion protein (PrPTSE) in extracellular vesicles from plasma of mice infected with mouse-adapted variant Creutzfeldt-Jakob disease by in vitro amplification.The Journal of biological chemistry · 2014Article
- Accumulation profiles of PrP(Sc) in hemal nodes of naturally and experimentally scrapie-infected sheep.BMC veterinary research · 2013Article
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- Identification of misfolded proteins in body fluids for the diagnosis of prion diseases.International journal of cell biology · 2013Review
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- Classical scrapie prions in ovine blood are associated with B lymphocytes and platelet-rich plasma.BMC veterinary research · 2011Article
- Sparse PrP(Sc) accumulation in the placentas of goats with naturally acquired scrapie.BMC veterinary research · 2011Article
- Prion disease blood test using immunoprecipitation and improved quaking-induced conversion.mBio · 2011Article
- Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays.PLoS pathogens · 2010Article
- Ovine serum biomarkers of early and late phase scrapie.BMC veterinary research · 2010Article
- Review
- Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of blood in the iatrogenic transmission of transmissible spongiform encephalopathy (TSE) or prion disease has become an increasing concern since the reports of variant Creutzfeldt-Jakob disease (vCJD) transmission through blood transfusion from humans with subclinical infection. The development of highly sensitive rapid assays to screen for prion infection in blood is of high priority in order to facilitate the prevention of transmission via blood and blood products. In the present study we show that PrP(sc), a surrogate marker for TSE infection, can be detected in cells isolated from the blood from naturally and experimentally infected sheep by using a rapid ligand-based immunoassay. In sheep with clinical disease, PrP(sc) was detected in the blood of 55% of scrapie agent-infected animals (n = 80) and 71% of animals with bovine spongiform encephalopathy (n = 7). PrP(sc) was also detected several months before the onset of clinical signs in a subset of scrapie agent-infected sheep, followed from 3 months of age to clinical disease. This study confirms that PrP(sc) is associated with the cellular component of blood and can be detected in preclinical sheep by an immunoassay in the absence of in vitro or in vivo amplification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.