ArticleNucleic acids research2009
Regulation of transcription termination in the nematode Caenorhabditis elegans.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Parameters that influence bipartite reporter system expression in Caenorhabditis elegans.Genetics · 2025Article
- Article
- Quantitative analysis of C. elegans transcripts by Nanopore direct-cDNA sequencing reveals terminal hairpins in non trans-spliced mRNAs.Nature communications · 2023Article
- Resolution of polycistronic RNA by SL2RNA (New York, N.Y.) · 2020Article
- Article
- Localization of RNAPII and 3' end formation factor CstF subunits on C. elegans genes and operons.Transcription · 2016Article
- Ending the message: poly(A) signals then and now.Genes & development · 2011Review
- Identification of transcription start sites of trans-spliced genes: uncovering unusual operon arrangements.RNA (New York, N.Y.) · 2011Article
- RNA polymerase II C-terminal domain phosphorylation patterns in Caenorhabditis elegans operons, polycistronic gene clusters with only one promoter.Molecular and cellular biology · 2010Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The current predicted mechanisms that describe RNA polymerase II (pol II) transcription termination downstream of protein expressing genes fail to adequately explain, how premature termination is prevented in eukaryotes that possess operon-like structures. Here we address this issue by analysing transcription termination at the end of single protein expressing genes and genes located within operons in the nematode Caenorhabditis elegans. By using a combination of RT-PCR and ChIP analysis we found that pol II generally transcribes up to 1 kb past the poly(A) sites into the 3' flanking regions of the nematode genes before it terminates. We also show that pol II does not terminate after transcription of internal poly(A) sites in operons. We provide experimental evidence that five randomly chosen C. elegans operons are transcribed as polycistronic pre-mRNAs. Furthermore, we show that cis-splicing of the first intron located in downstream positioned genes in these polycistronic pre-mRNAs is critical for their expression and may play a role in preventing premature pol II transcription termination.
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Registered trials
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