ArticleThe Journal of biological chemistry2009
A novel biosynthetic pathway of archaetidyl-myo-inositol via archaetidyl-myo-inositol phosphate from CDP-archaeol and D-glucose 6-phosphate in methanoarchaeon Methanothermobacter thermautotrophicus cells.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 37 citations in OpenAlex.
- Membrane lipid and expression responses ofFrontiers in microbiology · 2023Article
- The catalytic and structural basis of archaeal glycerophospholipid biosynthesis.Extremophiles : life under extreme conditions · 2022Review
- Open Issues for Protein Function Assignment inGenes · 2021Article
- The Cell Membrane ofInternational journal of molecular sciences · 2020Review
- Combined transcriptomics-metabolomics profiling of the heat shock response in the hyperthermophilic archaeon Pyrococcus furiosus.Extremophiles : life under extreme conditions · 2019Article
- Structural basis for phosphatidylinositol-phosphate biosynthesis.Nature communications · 2015Article
- From promiscuity to the lipid divide: on the evolution of distinct membranes in Archaea and Bacteria.Journal of molecular evolution · 2014Article
- Biosynthesis of archaeal membrane ether lipids.Frontiers in microbiology · 2014Review
- An uncharacterized member of the ribokinase family in Thermococcus kodakarensis exhibits myo-inositol kinase activity.The Journal of biological chemistry · 2013Article
- Phylogenomic investigation of phospholipid synthesis in archaea.Archaea (Vancouver, B.C.) · 2012Review
- On physical properties of tetraether lipid membranes: effects of cyclopentane rings.Archaea (Vancouver, B.C.) · 2012Review
- Crystal structure of a trapped catalytic intermediate suggests that forced atomic proximity drives the catalysis of mIPS.Biophysical journal · 2011Article
- Early evolution of membrane lipids: how did the lipid divide occur?Journal of molecular evolution · 2011Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Ether-type inositol phospholipids are ubiquitously distributed in Archaea membranes. The present paper describes a novel biosynthetic pathway of the archaeal inositol phospholipid. To study the biosynthesis of archaetidylinositol in vitro, we prepared two possible substrates: CDP-archaeol, which was chemically synthesized, and myo-[(14)C]inositol 1-phosphate, which was enzymatically prepared from [(14)C]glucose 6-phosphate with the inositol 1-phosphate (IP) synthase of this organism. The complete structure of the IP synthase reaction product was determined to be 1l-myo-inositol 1-phosphate, based on gas liquid chromatography with a chiral column. When the two substrates were incubated with the Methanothermobacter thermautotrophicus membrane fraction, archaetidylinositol phosphate (AIP) was formed along with a small amount of archaetidylinositol (AI). The two products were identified by fast atom bombardment-mass spectrometry and chemical analyses. AI was formed from AIP by incubation with the membrane fraction, but AIP was not formed from AI. This finding indicates that archaeal AI was synthesized from CDP-archaeol and d-glucose 6-phosphate via myo-inositol 1-phosphate and AIP. Although the relevant enzymes were not isolated, three enzymes are implied: IP synthase, AIP synthase, and AIP phosphatase. AIP synthase was homologous to yeast phosphatidylinositol synthase, and we confirmed AIP synthase activity by cloning the encoding gene (MTH1691) and expressing it in Escherichia coli. AIP synthase is a newly found member of the enzyme superfamily CDP-alcohol phosphatidyltransferase, which includes a wide range of enzymes that attach polar head groups to ester- and ether-type phospholipids of bacterial and archaeal origin. This is the first report of the biosynthesis of ether-type inositol phospholipids in Archaea.
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