ArticleStem cells and development2010
PiggyBac transposon-mediated, reversible gene transfer in human embryonic stem cells.
Article in Stem cells and development, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- An episomal DNA vector platform for the persistent genetic modification of pluripotent stem cells and their differentiated progeny.Stem cell reports · 2022Article
- Mammary-specific expression of Trim24 establishes a mouse model of human metaplastic breast cancer.Nature communications · 2021Article
- Characterization of the cHS4 insulator in mouse embryonic stem cells.FEBS open bio · 2020Article
- A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells.Scientific reports · 2020Article
- The impact of transposable element activity on therapeutically relevant human stem cells.Mobile DNA · 2019Review
- Liver-targeted gene therapy: Approaches and challenges.Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society · 2015Review
- piggyBac insertional mutagenesis screen identifies a role for nuclear RHOA in human ES cell differentiation.Stem cell reports · 2015Article
- Stem cell-mediated delivery of SPIO-loaded gold nanoparticles for the theranosis of liver injury and hepatocellular carcinoma.Nanotechnology · 2014Article
- A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells.PloS one · 2014Article
- Article
- Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes.Cell research · 2013Article
- LHX2 regulates the neural differentiation of human embryonic stem cells via transcriptional modulation of PAX6 and CER1.Nucleic acids research · 2013Article
- Insertional mutagenesis by a hybrid piggyBac and sleeping beauty transposon in the rat.Genetics · 2012Article
- Transposon-mediated BAC transgenesis in human ES cells.Nucleic acids research · 2012Article
- Increasing doublecortin expression promotes migration of human embryonic stem cell-derived neurons.Stem cells (Dayton, Ohio) · 2012Article
- Efficient derivation and genetic modifications of human pluripotent stem cells on engineered human feeder cell lines.Stem cells and development · 2012Article
- Chimeric piggyBac transposases for genomic targeting in human cells.Nucleic acids research · 2012Article
- Hyperactive piggyBac gene transfer in human cells and in vivo.Human gene therapy · 2012Article
- Using mouse models to study function of transcriptional factors in T cell development.Cell regeneration (London, England) · 2012Review
- Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Permanent and reversible genetic modifications are important approaches to study gene function in different cell types. They are also important for stem cell researchers to explore and test the therapeutic potential of stem cells. The piggyBac transposon from insects is a rising nonviral system that efficiently mutagenizes and mediates gene transfer into the mammalian genome. It is also characterized by its precise excision, leaving no trace sequence behind so that the genomic integrity of the mutated cell can be restored. Here, we use an optimized piggyBac transposon system to mediate gene transfer and expression of a bifunctional fluorescent reporter in human embryonic stem (ES) cells. We provide molecular evidence for transposase-mediated piggyBac integration events and functional evidence for successful expression of a transferred fluorescent protein genes in human ES cells and their in vitro differentiated derivatives. We also demonstrate that the integrated piggyBac transposon can be removed and an undisrupted insertion site can be restored, which implies potential applications for its use in gene therapy and genetics studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.