ArticleMolecular therapy : the journal of the American Society of Gene Therapy2009
Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 78 citations in OpenAlex.
- Regenerative medicine: current research and perspective in pediatric surgery.Pediatric surgery international · 2023Review
- Microphysiological system for studying contractile differences in young, active, and old, sedentary adult derived skeletal muscle cells.Aging cell · 2022Article
- Systemic cell therapy for muscular dystrophies : The ultimate transplantable muscle progenitor cell and current challenges for clinical efficacy.Stem cell reviews and reports · 2021Review
- Approaches to characterize the transcriptional trajectory of human myogenesis.Cellular and molecular life sciences : CMLS · 2021Review
- The authors reply: Comment on: "Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients" by Etienne et al.Journal of cachexia, sarcopenia and muscle · 2020Article
- Stem cell therapy for muscular dystrophies.The Journal of clinical investigation · 2020Review
- Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients.Journal of cachexia, sarcopenia and muscle · 2020Article
- miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation.Skeletal muscle · 2020Review
- Isolation and characterization of myogenic precursor cells from human cremaster muscle.Scientific reports · 2019Article
- Article
- Aldehyde Dehydrogenases: Not Just Markers, but Functional Regulators of Stem Cells.Stem cells international · 2019Review
- IL-1β- and IL-4-polarized macrophages have opposite effects on adipogenesis of intramuscular fibro-adipogenic progenitors in humans.Scientific reports · 2018Article
- Skeletal Muscle Regenerative Potential of Human MuStem Cells following Transplantation into Injured Mice Muscle.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Human myogenic reserve cells are quiescent stem cells that contribute to muscle regeneration after intramuscular transplantation in immunodeficient mice.Scientific reports · 2017Article
- Retinoic acid maintains human skeletal muscle progenitor cells in an immature state.Cellular and molecular life sciences : CMLS · 2017Article
- Disruption of mitochondrial quality control in peripheral artery disease: New therapeutic opportunities.Pharmacological research · 2017Review
- Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle.Stem cell reports · 2016Article
- Mesenchymal Stem/Stromal Cells Derived From a Reproductive Tissue Niche Under Oxidative Stress Have High Aldehyde Dehydrogenase Activity.Stem cell reviews and reports · 2016Article
- Aldehyde dehydrogenase 1A1 in stem cells and cancer.Oncotarget · 2016Review
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Authors and funding
10 authors at 9 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aldehyde dehydrogenase 1A1 (ALDH) activity is one hallmark of human bone marrow (BM), umbilical cord blood (UCB), and peripheral blood (PB) primitive progenitors presenting high reconstitution capacities in vivo. In this study, we have identified ALDH(+) cells within human skeletal muscles, and have analyzed their phenotypical and functional characteristics. Immunohistofluorescence analysis of human muscle tissue sections revealed rare endomysial cells. Flow cytometry analysis using the fluorescent substrate of ALDH, Aldefluor, identified brightly stained (ALDH(br)) cells with low side scatter (SSC(lo)), in enzymatically dissociated muscle biopsies, thereafter abbreviated as SMALD(+) (for skeletal muscle ALDH(+)) cells. Phenotypical analysis discriminated two sub-populations according to CD34 expression: SMALD(+)/CD34(-) and SMALD(+)/CD34(+) cells. These sub-populations did not initially express endothelial (CD31), hematopoietic (CD45), and myogenic (CD56) markers. Upon sorting, however, whereas SMALD(+)/CD34(+) cells developed in vitro as a heterogeneous population of CD56(-) cells able to differentiate in adipoblasts, the SMALD(+)/CD34(-) fraction developed in vitro as a highly enriched population of CD56(+) myoblasts able to form myotubes. Moreover, only the SMALD(+)/CD34(-) population maintained a strong myogenic potential in vivo upon intramuscular transplantation. Our results suggest that ALDH activity is a novel marker for a population of new human skeletal muscle progenitors presenting a potential for cell biology and cell therapy.
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