ArticleVeterinary research
Effects of growth conditions on biofilm formation by Actinobacillus pleuropneumoniae.
Article in Veterinary research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 82 citations in OpenAlex.
- Review
- Biofilm mitigation in hybrid chemical-biological upcycling of waste polymers.Frontiers in bioengineering and biotechnology · 2024Article
- The morphology and metabolic changes of Actinobacillus pleuropneumoniae during its growth as a biofilm.Veterinary research · 2023Article
- Actinobacillus pleuropneumoniae encodes multiple phase-variable DNA methyltransferases that control distinct phasevarions.Nucleic acids research · 2023Article
- Article
- Identification of FtpA, a Dps-Like Protein Involved in Anti-Oxidative Stress and Virulence in Actinobacillus pleuropneumoniae.Journal of bacteriology · 2022Article
- Involvement of theFrontiers in veterinary science · 2022Article
- Article
- Expression of a Shiga-Like Toxin during Plastic Colonization by Two Multidrug-Resistant Bacteria,Microorganisms · 2020Article
- Genome-wide screening of lipoproteins in Actinobacillus pleuropneumoniae identifies three antigens that confer protection against virulent challenge.Scientific reports · 2020Article
- Incorporation ofFrontiers in veterinary science · 2018Article
- The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence inFrontiers in cellular and infection microbiology · 2018Article
- Complete genome sequence of Citrobacter werkmanii strain BF-6 isolated from industrial putrefaction.BMC genomics · 2017Article
- Actinobacillus pleuropneumoniae grows as aggregates in the lung of pigs: is it time to refine our in vitro biofilm assays?Microbial biotechnology · 2017Article
- Auxotrophic Actinobacillus pleurpneumoniae grows in multispecies biofilms without the need for nicotinamide-adenine dinucleotide (NAD) supplementation.BMC microbiology · 2016Article
- Surface Polysaccharide Mutants Reveal that Absence of O Antigen Reduces Biofilm Formation of Actinobacillus pleuropneumoniae.Infection and immunity · 2016Article
- Concurrent host-pathogen gene expression in the lungs of pigs challenged with Actinobacillus pleuropneumoniae.BMC genomics · 2015Article
- Characterization of Biofilm Formation in [Pasteurella] pneumotropica and [Actinobacillus] muris Isolates of Mouse Origin.PloS one · 2015Article
- Role of (p)ppGpp in Viability and Biofilm Formation of Actinobacillus pleuropneumoniae S8.PloS one · 2015Article
- Biofilm formation by virulent and non-virulent strains of Haemophilus parasuis.Veterinary research · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilm formation is an important virulence trait of many bacterial pathogens. It has been reported in the literature that only two of the reference strains of the swine pathogen Actinobacillus pleuropneumoniae, representing serotypes 5b and 11, were able to form biofilm in vitro. In this study, we compared biofilm formation by the serotype 1 reference strain S4074 of A. pleuropneumoniae grown in five different culture media. We observed that strain S4074 of A. pleuropneumoniae is able to form biofilms after growth in one of the culture conditions tested brain heart infusion (BHI medium, supplier B). Confocal laser scanning microscopy using a fluorescent probe specific to the poly-N-acetylglucosamine (PGA) polysaccharide further confirmed biofilm formation. In accordance, biofilm formation was susceptible to dispersin B, a PGA hydrolase. Transcriptional profiles of A. pleuropneumoniae S4074 following growth in BHI-B, which allowed a robust biofilm formation, and in BHI-A, in which only a slight biofilm formation was observed, were compared. Genes such as tadC, tadD, genes with homology to autotransporter adhesins as well as genes pgaABC involved in PGA biosynthesis and genes involved in zinc transport were up-regulated after growth in BHI-B. Interestingly, biofilm formation was inhibited by zinc, which was found to be more present in BHI-A (no or slight biofilm) than in BHI-B. We also observed biofilm formation in reference strains representing serotypes 3, 4, 5a, 12 and 14 as well as in 20 of the 37 fresh field isolates tested. Our data indicate that A. pleuropneumoniae has the ability to form biofilms under appropriate growth conditions and transition from a biofilm-positive to a biofilm-negative phenotype was reversible.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.