ArticleMolecular microbiology2009
The right half of the Escherichia coli replication origin is not essential for viability, but facilitates multi-forked replication.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Robust helicase loading mechanism underlies diverse architectures of bacterial replication origins.Nucleic acids research · 2026Article
- The Escherichia coli replication initiator DnaA is titrated on the chromosome.Nature communications · 2025Article
- Adaptive evolution of oriC through in vitro propagation of a mini-chromosome in RCR.Nucleic acids research · 2025Article
- Article
- IHF and Fis asInternational journal of molecular sciences · 2023Review
- Concerted actions of DnaA complexes with DNA-unwinding sequences within and flanking replication origin oriC promote DnaB helicase loading.The Journal of biological chemistry · 2022Article
- Blocking, Bending, and Binding: Regulation of Initiation of Chromosome Replication During theFrontiers in microbiology · 2021Review
- Identification of a basal system for unwinding a bacterial chromosome origin.The EMBO journal · 2019Article
- The bacterial cell cycle, chromosome inheritance and cell growth.Nature reviews. Microbiology · 2019Review
- Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation.Frontiers in microbiology · 2019Review
- Article
- Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome replication.Nucleic acids research · 2018Article
- Structure and Function of theFrontiers in microbiology · 2018Article
- Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach.Journal of visualized experiments : JoVE · 2017Article
- The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators.Nucleic acids research · 2017Article
- Review
- Targeting the Bacterial Orisome in the Search for New Antibiotics.Frontiers in microbiology · 2017Article
- Inactivation of Individual SeqA Binding Sites of the E. coli Origin Reveals Robustness of Replication Initiation Synchrony.PloS one · 2016Article
- Opening the Strands of Replication Origins-Still an Open Question.Frontiers in molecular biosciences · 2016Review
- Regulation of DNA Replication Initiation by Chromosome Structure.Journal of bacteriology · 2015Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
Replication initiation is a key event in the cell cycle of all organisms and oriC, the replication origin in Escherichia coli, serves as the prototypical model for this process. The minimal sequence required for oriC function was originally determined entirely from plasmid studies using cloned origin fragments, which have previously been shown to differ dramatically in sequence requirement from the chromosome. Using an in vivo recombineering strategy to exchange wt oriCs for mutated ones regardless of whether they are functional origins or not, we have determined the minimal origin sequence that will support chromosome replication. Nearly the entire right half of oriC could be deleted without loss of origin function, demanding a reassessment of existing models for initiation. Cells carrying the new DnaA box-depleted 163 bp minimal oriC exhibited little or no loss of fitness under slow-growth conditions, but were sensitive to rich medium, suggesting that the dense packing of initiator binding sites that is a hallmark of prokaryotic origins, has likely evolved to support the increased demands of multi-forked replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.