ArticleLangmuir : the ACS journal of surfaces and colloids2010
Nanofabricated racks of aligned and anchored DNA substrates for single-molecule imaging.
Article in Langmuir : the ACS journal of surfaces and colloids, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Single-molecule imaging reveals DNA shape read-out by the INO80 chromatin remodeler.Nature communications · 2026Article
- Single-molecule DNA-flow stretching assay as a versatile hybrid tool for investigating DNA-protein interactions.BMB reports · 2025Review
- Cell shape controls rheotaxis in small parasitic bacteria.PLoS pathogens · 2022Article
- Single-molecule fluorescence imaging techniques reveal molecular mechanisms underlying deoxyribonucleic acid damage repair.Frontiers in bioengineering and biotechnology · 2022Review
- Molecular mechanisms ofBiophysics reports · 2021Article
- Oriented Soft DNA Curtains for Single-Molecule Imaging.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- Single-Stranded DNA Curtains for Single-Molecule Visualization of Rad51-ssDNA Filament Dynamics.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Campylobacter jejuni motility integrates specialized cell shape, flagellar filament, and motor, to coordinate action of its opposed flagella.PLoS pathogens · 2020Article
- Diffusion of DNA-Binding Species in the Nucleus: A Transient Anomalous Subdiffusion Model.Biophysical journal · 2020Article
- Single-molecule visualization of human BLM helicase as it acts upon double- and single-stranded DNA substrates.Nucleic acids research · 2019Article
- DNA skybridge: 3D structure producing a light sheet for high-throughput single-molecule imaging.Nucleic acids research · 2019Article
- Single-Stranded DNA Curtains for Studying the Srs2 Helicase Using Total Internal Reflection Fluorescence Microscopy.Methods in enzymology · 2018Article
- Single-Stranded DNA Curtains for Studying Homologous Recombination.Methods in enzymology · 2017Article
- Visualizing recombination intermediates with single-stranded DNA curtains.Methods (San Diego, Calif.) · 2016Review
- High-Throughput Universal DNA Curtain Arrays for Single-Molecule Fluorescence Imaging.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- The dynamics of eukaryotic replication initiation: origin specificity, licensing, and firing at the single-molecule level.Molecular cell · 2015Article
- High-throughput single-molecule studies of protein-DNA interactions.FEBS letters · 2014Review
- Visualizing protein movement on DNA at the single-molecule level using DNA curtains.DNA repair · 2014Review
- Direct optical mapping of transcription factor binding sites on field-stretched λ-DNA in nanofluidic devices.Nucleic acids research · 2014Article
- DNA dynamics and single-molecule biology.Chemical reviews · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Single-molecule studies of biological macromolecules can benefit from new experimental platforms that facilitate experimental design and data acquisition. Here we develop new strategies to construct curtains of DNA in which the molecules are aligned with respect to one another and maintained in an extended configuration by anchoring both ends of the DNA to the surface of a microfluidic sample chamber that is otherwise coated with an inert lipid bilayer. This "double-tethered" DNA substrate configuration is established through the use of nanofabricated rack patterns comprised of two distinct functional elements: linear barriers to lipid diffusion that align DNA molecules anchored by one end to the bilayer and antibody-coated pentagons that provide immobile anchor points for the opposite ends of the DNA. These devices enable the alignment and anchoring of thousands of individual DNA molecules, which can then be visualized using total internal reflection fluorescence microscopy under conditions that do not require continuous application of buffer flow to stretch the DNA. This unique strategy offers the potential for studying protein-DNA interactions on large DNA substrates without compromising measurements through application of hydrodynamic force. We provide a proof-of-principle demonstration that double-tethered DNA curtains made with nanofabricated rack patterns can be used in a one-dimensional diffusion assay that monitors the motion of quantum dot-tagged proteins along DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.